Takahisa Suzuki1, Naoki Iwamoto2, Satoshi Yamasaki1, Ayako Nishino1, Yoshikazu Nakashima1, Yoshiro Horai1, Shin-Ya Kawashiri1, Kunihiro Ichinose1, Kazuhiko Arima1, Mami Tamai1, Hideki Nakamura1, Tomoki Origuchi1, Chikara Miyamoto1, Makoto Osaki1, Kaname Ohyama1, Naotaka Kuroda1, Atsushi Kawakami1. 1. From the Department of Immunology and Rheumatology, Department of Public Health, Department of Health Sciences, Department of Orthopedic Surgery, and Department of Environmental and Pharmaceutical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki; and Department of Clinical Immunology and Rheumatology, Hiroshima University, Hiroshima, Japan.T. Suzuki, MD; N. Iwamoto, MD, PhD, Assistant Professor, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; S. Yamasaki, MD, PhD, Department of Clinical Immunology and Rheumatology, Hiroshima University; A. Nishino, MD; Y. Nakashima, MD; Y. Horai, MD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; S.Y. Kawashiri, MD, PhD, Department of Immunology and Rheumatology, and Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences; K. Ichinose, MD, PhD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; K. Arima, MD, PhD, Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences; M. Tamai, MD, PhD; H. Nakamura, MD, PhD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; T. Origuchi, MD, PhD, Professor, Department of Health Sciences, Nagasaki University Graduate School of Biomedical Sciences; C. Miyamoto, MD, PhD; M. Osaki, MD, PhD, Professor, Department of Orthopedic Surgery, Nagasaki University Graduate School of Biomedical Sciences; K. Ohyama, PhD; N. Kuroda, PhD, Professor, Department of Environmental and Pharmaceutical Sciences, Nagasaki University Graduate School of Biomedical Sciences; A. Kawakami, MD, PhD, Professor, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences. 2. From the Department of Immunology and Rheumatology, Department of Public Health, Department of Health Sciences, Department of Orthopedic Surgery, and Department of Environmental and Pharmaceutical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki; and Department of Clinical Immunology and Rheumatology, Hiroshima University, Hiroshima, Japan.T. Suzuki, MD; N. Iwamoto, MD, PhD, Assistant Professor, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; S. Yamasaki, MD, PhD, Department of Clinical Immunology and Rheumatology, Hiroshima University; A. Nishino, MD; Y. Nakashima, MD; Y. Horai, MD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; S.Y. Kawashiri, MD, PhD, Department of Immunology and Rheumatology, and Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences; K. Ichinose, MD, PhD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; K. Arima, MD, PhD, Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences; M. Tamai, MD, PhD; H. Nakamura, MD, PhD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; T. Origuchi, MD, PhD, Professor, Department of Health Sciences, Nagasaki University Graduate School of Biomedical Sciences; C. Miyamoto, MD, PhD; M. Osaki, MD, PhD, Professor, Department of Orthopedic Surgery, Nagasaki University Graduate School of Biomedical Sciences; K. Ohyama, PhD; N. Kuroda, PhD, Professor, Department of Environmental and Pharmaceutical Sciences, Nagasaki University Graduate School of Biomedical Sciences; A. Kawakami, MD, PhD, Professor, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences. naoki-iwa@nagasaki-u.ac.jp.
Abstract
OBJECTIVE: To investigate the role of thrombospondin 1 (TSP-1) in RA. METHODS: Expression of TSP-1 in synovial tissues was determined by immunohistochemistry. Expression of TSP-1 in rheumatoid fibroblast-like synovial cells (FLS) was investigated by quantitative real-time PCR and ELISA. Correlations among the plasma TSP-1 and other variables in patients with RA were examined. RESULTS: Expression of TSP-1 was increased in rheumatoid synovial tissues. Transforming growth factor-β1 (TGF-β1) clearly increased TSP-1 expression in FLS on both mRNA and protein levels. Changes in plasma TSP-1 were associated with those in 28-joint Disease Activity Score-erythrocyte sedimentation rate and plasma TGF-β1. CONCLUSION: TSP-1 might be critically involved in the disease process of RA through the TGF-β1/TSP-1 axis.
OBJECTIVE: To investigate the role of thrombospondin 1 (TSP-1) in RA. METHODS: Expression of TSP-1 in synovial tissues was determined by immunohistochemistry. Expression of TSP-1 in rheumatoid fibroblast-like synovial cells (FLS) was investigated by quantitative real-time PCR and ELISA. Correlations among the plasma TSP-1 and other variables in patients with RA were examined. RESULTS: Expression of TSP-1 was increased in rheumatoid synovial tissues. Transforming growth factor-β1 (TGF-β1) clearly increased TSP-1 expression in FLS on both mRNA and protein levels. Changes in plasma TSP-1 were associated with those in 28-joint Disease Activity Score-erythrocyte sedimentation rate and plasma TGF-β1. CONCLUSION:TSP-1 might be critically involved in the disease process of RA through the TGF-β1/TSP-1 axis.
Authors: Gautam Edhayan; Ray A Ohara; W Alex Stinson; M Asif Amin; Takeo Isozaki; Christine M Ha; G Kenneth Haines; Rachel Morgan; Phillip L Campbell; Ali S Arbab; Sean C Friday; David A Fox; Jeffrey H Ruth Journal: Arthritis Res Ther Date: 2016-04-12 Impact factor: 5.156
Authors: Michal A Rahat; Mirna Safieh; Elina Simanovich; Eliran Pasand; Tal Gazitt; Amir Haddad; Muna Elias; Devy Zisman Journal: Arthritis Res Ther Date: 2020-10-14 Impact factor: 5.156