Correlation between polymorphisms of the E-selectin gene, hepatitis B virus DNA copies, pre-S1 antigen and clinical outcomes during chronic hepatitis B.

The aim of this study was to investigate the relationships between E-selectin +G98T, +A561C polymorphisms and different progression in Hepatitis B virus (HBV) infection Xinjiang Han population, also to determine the HBV DNA copies and pre-S1 antigen (preS1Ag) in this population. Polymorphisms of the E-selectin gene in 200 chronic HBV infection (61 cases of chronic HBV carriers, chronic hepatitis B 75, liver cirrhosis 43, liver cancer 21) and 200 healthy controls were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Real-time quantitative PCR was used to detect the levels of HBV DNA. preS1Ag and five items of hepatitis B were detected by enzyme-linked immunosorbent assay. Liver fibrosis using chemiluminescence, biochemical markers using Roche 7600 automatic biochemical analyzer. E-selectin +A561C polymorphism of A/C genotype and C allele frequency in chronic hepatitis B (CHB) and cirrhosis (LC) group were compared with the control group had significant difference (P < 0.05). The risk of CHB and LC, AC genotype were 2.09, 2.33 times of the AA genotype. In group of CHB, the levels of HBV DNA and preS1Ag in the AC genotype patients were higher than those in the AA genotype (P < 0.05). However, there were no significant differences in comparison of liver function and liver fibrosis index in different genotypes of CHB and LC group. +A561C and +G98T linkage disequilibrium analysis showed: D' = 0.632, r(2) = 0.202, haplotype analysis showed that the G-A haplotype OR = 0.507, G-C haplotype OR = 1.973. E-selectin +A561C polymorphism may have some correlation with the occurrence of CHB and LC, and allele C may be one of the predisposing factors. AC polymorphism may affect HBV replication in CHB, but may not play an important and direct effect on liver injury and liver fibrosis after HBV infection. There were some linkage of +A561C and +G98T, G-C haplotype may be a risk factor for chronic HBV infection.