Suleyman Cetinkunar1, Serhat Tokgoz2, Bulent Caglar Bilgin3, Hasan Erdem1, Recep Aktimur4, Serpil Can5, Huseyin Serkan Erol6, Atilla Isgoren7, Selim Sozen8, Yilmaz Polat9. 1. Adana Numune Egitim ve Arastirma Hastanesi, Genel Cerrahi Klinigi Adana, Türkiye. 2. Ankara Diskapi Yildirim Beyazit Egitim ve Arastirma Hastanesi, Genel Cerrahi Klinigi Ankara, Türkiye. 3. Kafkas Universitesi Tip Fakultesi, Genel Cerrahi Ana Bilim Dali Kars, Türkiye. 4. Samsun Egitim ve Arastirma Hastanesi, Genel Cerrahi Klinigi Samsun, Türkiye. 5. Kafkas Universitesi Tip Fakultesi, Fizyoloji Ana Bilim Dali Kars, Türkiye. 6. Ataturk Universitesi Veterinerlik Fakultesi, Biyokimya Ana Bilim Dali Erzurum, Türkiye. 7. Ankara Universtesi, Veterinerlik Fakultesi Ankara, Türkiye. 8. Namik Kemal Universitesi Tip Fakultesi, Genel Cerrahi Ana Bilim Dali Tekirdag, Türkiye. 9. Medikal Park Hastanesi, Genel Cerrahi Klinigi Elazig, Turkiye.
Abstract
AIM: Silymarin from Silybum marianum was found to reduce liver injury. The aim of the present study was to investigate the effects of silymarin on hepatic regeneration in partially hepatectomized rats. METHODS: Thirty Wistar-Albino rats were divided into 3 groups of 10 animals as sham, control and experimental groups. In the sham group (n=10) abdominal incision was closed after laparotomy. In the control group (n=10), the rats underwent 70% hepatectomy after laparotomy. In the experimental group (n=10) after partial 70% hepatectomy, silymarin (200 mg/kg/d) were given to rats for 10 days. Rats in three groups were sacrificed on 10 days. Aspartate (AST) and alanine transaminase (ALT), gamma glutamyl transferase (GGT), ALP, LDH and total bilirubin levels were measured using intracardiac blood samples. Tissue malondialdehyde (MDA) and tissue glutathion (GSH) and Superoxide dismutase (SOD) levels were measured. To reveal the increase in the mass of the remnant liver tissue in the control and experimental groups relative weight of the liver was calculated. Histopathological analysis of the liver was performed using a semi-quantitative scoring system. RESULTS: A statistically significant difference among three groups was not shown for AST and ALT levels. A statistically significant difference was found between the groups as for total bilirubin and gamma glutamyl transferase levels. Increases in relative liver weights were seen with time in Groups 2 and 3. A statistically significant difference was not found for tissue malondialdehyde, Glutathion and Superoxide dismutase levels between hepatectomy and hepatectomy + silymarin groups. On liver tissue sections of the rats in the hepatectomy + silymarin group, increased regeneration and lipid peroxidation were observed accompanied by decreased antioxidant response. CONCLUSION: It has been observed that silymarin with many established functions such as antiproliferative, anti-inflammatory and energy antioxidant effects, does not contributed to proliferative regeneration of the liver-which has very important metabolic functions -after partial hepatectomy; instead it will decrease serum levels of transaminases.
AIM: Silymarin from Silybum marianum was found to reduce liver injury. The aim of the present study was to investigate the effects of silymarin on hepatic regeneration in partially hepatectomized rats. METHODS: Thirty Wistar-Albino rats were divided into 3 groups of 10 animals as sham, control and experimental groups. In the sham group (n=10) abdominal incision was closed after laparotomy. In the control group (n=10), the rats underwent 70% hepatectomy after laparotomy. In the experimental group (n=10) after partial 70% hepatectomy, silymarin (200 mg/kg/d) were given to rats for 10 days. Rats in three groups were sacrificed on 10 days. Aspartate (AST) and alanine transaminase (ALT), gamma glutamyl transferase (GGT), ALP, LDH and total bilirubin levels were measured using intracardiac blood samples. Tissue malondialdehyde (MDA) and tissue glutathion (GSH) and Superoxide dismutase (SOD) levels were measured. To reveal the increase in the mass of the remnant liver tissue in the control and experimental groups relative weight of the liver was calculated. Histopathological analysis of the liver was performed using a semi-quantitative scoring system. RESULTS: A statistically significant difference among three groups was not shown for AST and ALT levels. A statistically significant difference was found between the groups as for total bilirubin and gamma glutamyl transferase levels. Increases in relative liver weights were seen with time in Groups 2 and 3. A statistically significant difference was not found for tissue malondialdehyde, Glutathion and Superoxide dismutase levels between hepatectomy and hepatectomy + silymarin groups. On liver tissue sections of the rats in the hepatectomy + silymarin group, increased regeneration and lipid peroxidation were observed accompanied by decreased antioxidant response. CONCLUSION: It has been observed that silymarin with many established functions such as antiproliferative, anti-inflammatory and energy antioxidant effects, does not contributed to proliferative regeneration of the liver-which has very important metabolic functions -after partial hepatectomy; instead it will decrease serum levels of transaminases.
Authors: Frank J Burczynski; Guqi Wang; David Nguyen; Yufei Chen; Howard J Smith; Yuewen Gong Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban Date: 2012-01