Hao Guan1, Dahai Hu1, Zhijing Zhao2, Weixia Cai1, Qin Zhou1, Ximing Yang3, Ying Yan4, Xiongxiang Zhu1. 1. Department of Burn, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shanxi, China. 2. Department of Cardiology, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shanxi, China. 3. Department of Burn, Yan'an University Affiliated Hospital, Yanan University Yan'an 710032, Shanxi, China. 4. Department of Burn, 413 Hospital of People's Liberation Army Zhoushan 316000, Zhejiang, China.
Abstract
BACKGROUND: Endothelial recovery is a central feature of tissues after frostbite injuries. Thermo tolerance plays an important role in protecting cells against injury after frozen and thawing. The present study aimed to quantitatively assess the injury of human umbilical vein endothelial cells HUVEC-12 after repeated low temperature. MATERIAL AND METHOD: Pretreatments (HUVEC-12) cells were repeatedly exposed to cold (1°C/min decrement to -20°C). Their proliferation, death, apoptosis, and protein and mRNA expressions of HSP70 were determined. RESULTS: Endothelial cells after repeated cold exposures were more resistant to apoptosis and necrosis than normal cells. The expressions of HSP70 in cells after repeated cold exposures were significantly higher than in normal HUVEC-12 cells (P < 0.05). CONCLUSION: Cold acclimation may induce the expression of HSP-70 which plays a protective role in the temperature tolerance.
BACKGROUND: Endothelial recovery is a central feature of tissues after frostbite injuries. Thermo tolerance plays an important role in protecting cells against injury after frozen and thawing. The present study aimed to quantitatively assess the injury of human umbilical vein endothelial cells HUVEC-12 after repeated low temperature. MATERIAL AND METHOD: Pretreatments (HUVEC-12) cells were repeatedly exposed to cold (1°C/min decrement to -20°C). Their proliferation, death, apoptosis, and protein and mRNA expressions of HSP70 were determined. RESULTS: Endothelial cells after repeated cold exposures were more resistant to apoptosis and necrosis than normal cells. The expressions of HSP70 in cells after repeated cold exposures were significantly higher than in normal HUVEC-12 cells (P < 0.05). CONCLUSION: Cold acclimation may induce the expression of HSP-70 which plays a protective role in the temperature tolerance.
Entities:
Keywords:
Temperature; heat shock protein 70; human umbilical vein endothelial cells-12; thawing
Authors: Susanna Airaksinen; Terhi Jokilehto; Christina M I Råbergh; Mikko Nikinmaa Journal: Comp Biochem Physiol B Biochem Mol Biol Date: 2003-10 Impact factor: 2.231