Ning Yao1, Ping Yan1, Rong-Fu Wang1, Chun-Li Zhang1, Chao Ma1, Xue-Qi Chen1, Qian Zhao2, Pan Hao1. 1. Department of Nuclear Medicine, Peking University First Hospital West District, Beijing 100034, China. 2. Department of Nuclear Medicine, General Hospital of Ningxia Medical University Xingqing District, Yinchuan 750004, Ningxia, China.
Abstract
BACKGROUND: To improve the detection of pulmonary metastases, experimental blood-borne pulmonary metastasis mouse models were established using three intravenously administered cell lines. In a previous study we demonstrated that (99m)Tc-radiolabeled arginine-arginine-leucine (RRL) could be used to non-invasively image malignant tumors. METHODS: (99m)Tc-RRL was prepared and injected intravenously in mice with pulmonary metastases that arose from the intravenous injection of HepG2, B16, and Hela cells. The bio-distribution and imaging of (99m)Tc-RRL were determined in different pulmonary metastases mouse models and in normal mice. RESULTS: (99m)Tc-RRL exhibited higher uptake values in the lungs of pulmonary metastatic mice compared to normal mice (P<0.05; 3.92±0.48% ID/g 2 h post-injection and 3.89±0.36% ID/g 4 h post-injection in metastatic hepatic carcinoma [HepG2]-bearing lungs; 5.49±0.84% ID/g 2 h post-injection and 5.11±0.75% ID/g 4 h post-injection in metastatic melanoma [B16]-bearing lungs; 3.72±0.52% ID/g 2 h post-injection and 3.51±0.35% ID/g 4 h post-injection in metastatic cervical carcinoma [Hela]-bearing lungs; 2.38±0.20% ID/g 2 h post-injection and 2.11±0.24% ID/g 4 h post-injection in normal lungs). The pulmonary metastatic lesions were clearly visualized using (99m)Tc-RRL. CONCLUSIONS: (99m)Tc-RRL exhibited favorable metastatic tumor targeting and imaging properties, thus highlighting its potential as an effective imaging probe for detection of pulmonary metastases. (99m)Tc-RRL can be used as a reasonable supplement to (18)F-FDG imaging in the non-invasive imaging of tumor angiogenesis.
BACKGROUND: To improve the detection of pulmonary metastases, experimental blood-borne pulmonary metastasismouse models were established using three intravenously administered cell lines. In a previous study we demonstrated that (99m)Tc-radiolabeled arginine-arginine-leucine (RRL) could be used to non-invasively image malignant tumors. METHODS: (99m)Tc-RRL was prepared and injected intravenously in mice with pulmonary metastases that arose from the intravenous injection of HepG2, B16, and Hela cells. The bio-distribution and imaging of (99m)Tc-RRL were determined in different pulmonary metastasesmouse models and in normal mice. RESULTS: (99m)Tc-RRL exhibited higher uptake values in the lungs of pulmonary metastatic mice compared to normal mice (P<0.05; 3.92±0.48% ID/g 2 h post-injection and 3.89±0.36% ID/g 4 h post-injection in metastatic hepatic carcinoma [HepG2]-bearing lungs; 5.49±0.84% ID/g 2 h post-injection and 5.11±0.75% ID/g 4 h post-injection in metastatic melanoma [B16]-bearing lungs; 3.72±0.52% ID/g 2 h post-injection and 3.51±0.35% ID/g 4 h post-injection in metastatic cervical carcinoma [Hela]-bearing lungs; 2.38±0.20% ID/g 2 h post-injection and 2.11±0.24% ID/g 4 h post-injection in normal lungs). The pulmonary metastatic lesions were clearly visualized using (99m)Tc-RRL. CONCLUSIONS: (99m)Tc-RRL exhibited favorable metastatic tumor targeting and imaging properties, thus highlighting its potential as an effective imaging probe for detection of pulmonary metastases. (99m)Tc-RRL can be used as a reasonable supplement to (18)F-FDG imaging in the non-invasive imaging of tumor angiogenesis.
Authors: Gregory E R Weller; Michael K K Wong; Ruth A Modzelewski; Erxiong Lu; Alexander L Klibanov; William R Wagner; Flordeliza S Villanueva Journal: Cancer Res Date: 2005-01-15 Impact factor: 12.701