BACKGROUND: There are several standard diets for animals used in scientific research, usually conceived by scientific institutions. The AIN-93 diet is widely used, but there are some reports of fatty liver in Wistar rats fed this diet. OBJECTIVE: We aimed to evaluate the hepatic repercussions of the AIN-93 diet intake in Wistar rats. METHODS: Forty newly-weaned 21-day-old male Wistar rats were fed either the AIN-93 diet or a commercial diet for either 1 month or 4 months. Weight gain, serum biochemistry, hepatic histology, and hepatic fatty acid profile were analyzed. RESULTS: Hepatic steatosis was observed, especially in the group fed the AIN-93 diet. Serum blood glucose, absolute and relative liver weight and hepatic levels of oleic, palmitoleic, stearic, and palmitic fatty acids were related to the observed steatosis, while lipidogram and serum markers of liver function and injury were not. CONCLUSION: AIN-93 diet induced acute hepatic steatosis in Wistar rats, which may compromise its use as a standard diet for experimental studies with rodents. The hepatic fatty acid profile was associated with steatosis, with possible implications for disease prognosis. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.
BACKGROUND: There are several standard diets for animals used in scientific research, usually conceived by scientific institutions. The AIN-93 diet is widely used, but there are some reports of fatty liver in Wistar rats fed this diet. OBJECTIVE: We aimed to evaluate the hepatic repercussions of the AIN-93 diet intake in Wistar rats. METHODS: Forty newly-weaned 21-day-old male Wistar rats were fed either the AIN-93 diet or a commercial diet for either 1 month or 4 months. Weight gain, serum biochemistry, hepatic histology, and hepatic fatty acid profile were analyzed. RESULTS:Hepatic steatosis was observed, especially in the group fed the AIN-93 diet. Serum blood glucose, absolute and relative liver weight and hepatic levels of oleic, palmitoleic, stearic, and palmitic fatty acids were related to the observed steatosis, while lipidogram and serum markers of liver function and injury were not. CONCLUSION: AIN-93 diet induced acute hepatic steatosis in Wistar rats, which may compromise its use as a standard diet for experimental studies with rodents. The hepatic fatty acid profile was associated with steatosis, with possible implications for disease prognosis. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.
Authors: Juliana Célia F Santos; Orlando R P de Araújo; Iara B Valentim; Kívia Queiroz de Andrade; Fabiana Andréa Moura; Salete Smaniotto; John Marques dos Santos; Juciano Gasparotto; Daniel P Gelain; Marília O F Goulart Journal: Oxid Med Cell Longev Date: 2015-06-02 Impact factor: 6.543
Authors: Sophie B Chehade; George B H Green; Christopher D Graham; Ayanabha Chakraborti; Bijal Vashai; Amber Moon; Michael B Williams; Benjamin Vickers; Taylor Berryhill; William Van Der Pol; Landon Wilson; Mickie L Powell; Daniel L Smith; Stephen Barnes; Casey Morrow; M Shahid Mukhtar; Gregory D Kennedy; James A Bibb; Stephen A Watts Journal: Front Nutr Date: 2022-08-29