| Literature DB >> 25916483 |
Christoph Gertler1,2, Rafael Bargiela3, Francesca Mapelli4,5, Xifang Han6, Jianwei Chen6, Tran Hai7, Ranya A Amer8, Mouna Mahjoubi9, Hanan Malkawi10, Mirko Magagnini11, Ameur Cherif9, Yasser R Abdel-Fattah8, Nicolas Kalogerakis12, Daniele Daffonchio4,5, Manuel Ferrer3, Peter N Golyshin7.
Abstract
Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria.Entities:
Keywords: Alcanivorax; Bioremediation; Crude oil degradation
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Year: 2015 PMID： 25916483 DOI： 10.1007/s00248-015-0606-7
Source DB: PubMed Journal: Microb Ecol ISSN： 0095-3628 Impact factor: 4.552