Literature DB >> 2591568

Treatment of human spermatozoa with an egg yolk medium can enhance the outcome of in vitro fertilization.

K P Katayama1, E Stehlik, M Roesler, R S Jeyendran, W J Holmgren, L J Zaneveld.   

Abstract

In at least 4 of 7 cases, fertilization of intact human oocytes was more successful when spermatozoa were pretreated with TEST yolk medium at 5 degrees C for 2 hours as compared with the standard treatment with Ham's F-10 only. Both pregnancies that were obtained after the transfer of the fertilized oocytes resulted from oocytes fertilized by TEST yolk-treated spermatozoa. No decrease in fertilization occurred in any of the cases after TEST yolk treatment. If these results hold true for a larger series of patients, it may be worthwhile for the standard IVF incubation system of spermatozoa to include TEST yolk.

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Year:  1989        PMID： 2591568     DOI： 10.1016/s0015-0282(16)53181-1

Source DB:  PubMed          Journal:  Fertil Steril        ISSN： 0015-0282            Impact factor:   7.329


  6 in total

1.  Treatment of severe male-factor infertility with high concentrations of motile sperm by microinsemination in embryo cryopreservation straws.

Authors:  D G Hammitt; D L Walker; C H Syrop; T M Miller; M R Bennett
Journal:  J In Vitro Fert Embryo Transf       Date:  1991-04

2.  Biological pH buffers in IVF: help or hindrance to success.

Authors:  Matthew A Will; Natalie A Clark; Jason E Swain
Journal:  J Assist Reprod Genet       Date:  2011-05-26       Impact factor: 3.412

3.  Heat-induced hyperactivation.

Authors:  P J Chan; J U Corselli; W C Patton; J D Jacobson; A King
Journal:  J Assist Reprod Genet       Date:  1998-01       Impact factor: 3.412

4.  Egg yolk enhances the receptiveness of zona pellucida to sperm binding.

Authors:  D E Johnson; W J Holmgren; R S Jeyendran
Journal:  J Assist Reprod Genet       Date:  1994-08       Impact factor: 3.412

5.  Effects of preservation of mouse spermatozoa in electrolyte-free solution at 4 degrees C on the outcome of mouse in vitro fertilization.

Authors:  S Quan; S Yamano; H Nakasaka; K Hinokio; K Nagagawa; T Aono
Journal:  J Assist Reprod Genet       Date:  2000-08       Impact factor: 3.412

6.  The effect of a two-hour, room temperature incubation of human spermatozoa in TEST-yolk buffer on the rate of fertilization in vitro.

Authors:  Julie R Jensen; John H Walker; Amin A Milki; Lynn Westphal; Barry Behr
Journal:  J Assist Reprod Genet       Date:  2004-05       Impact factor: 3.412
  6 in total

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