Wei-Dong Lu1, Yun Zuo1, Zhen Xu1, Min Zhang1. 1. Wei-Dong Lu, Yun Zuo, Zhen Xu, Min Zhang, Department of Oncology, Zhangjiagang First People's Hospital, Suzhou University, Zhangjiagang 215600, Jiangsu Province, China.
Abstract
AIM: To investigate the mechanism by which miR-19a is up-regulated in gastric cancer (GC), which plays an oncogenic role. METHODS: In the present study, we investigated the role of miR-19a in gastric tissues as well as two GC cell lines. In vivo, we detected the basal expression level of miR-19a using real-time reverse transcription-PCR (RT-PCR), and the relevance between expression of miR-19a and clinicopathological information was analyzed. In vitro, miR-19a was ectopically expressed using overexpression and knock-down strategies. RESULTS: Overexpression of miR-19a was significantly associated with metastasis of GC and inferior overall prognosis. However, no significant correlation was found between miR-19a expression and other characteristics such as age, gender, tobacco, alcohol or tumor size. Cell proliferation, migration and invasion assays showed that overexpression of miR-19a promoted the proliferation, migration and invasion, and that overexpression of miR-19a promoted the epithelial-mesenchymal transition through activating the PI3K/AKT pathway. Blocking the PI3K/AKT pathway could cancel the effect of miR-19a. CONCLUSION: All together, our results suggest that miR-19a could be used as a promising therapeutic target in the treatment of GC.
AIM: To investigate the mechanism by which miR-19a is up-regulated in gastric cancer (GC), which plays an oncogenic role. METHODS: In the present study, we investigated the role of miR-19a in gastric tissues as well as two GC cell lines. In vivo, we detected the basal expression level of miR-19a using real-time reverse transcription-PCR (RT-PCR), and the relevance between expression of miR-19a and clinicopathological information was analyzed. In vitro, miR-19a was ectopically expressed using overexpression and knock-down strategies. RESULTS: Overexpression of miR-19a was significantly associated with metastasis of GC and inferior overall prognosis. However, no significant correlation was found between miR-19a expression and other characteristics such as age, gender, tobacco, alcohol or tumor size. Cell proliferation, migration and invasion assays showed that overexpression of miR-19a promoted the proliferation, migration and invasion, and that overexpression of miR-19a promoted the epithelial-mesenchymal transition through activating the PI3K/AKT pathway. Blocking the PI3K/AKT pathway could cancel the effect of miR-19a. CONCLUSION: All together, our results suggest that miR-19a could be used as a promising therapeutic target in the treatment of GC.
Authors: Tineke E Buffart; Beatriz Carvalho; Nicole C T van Grieken; Wessel N van Wieringen; Marianne Tijssen; Elma Meershoek-Klein Kranenbarg; Henk M W Verheul; Heike I Grabsch; Bauke Ylstra; Cornelis J H van de Velde; Gerrit A Meijer Journal: Oncologist Date: 2012-04-24
Authors: Mingxia Xiong; Lei Jiang; Yang Zhou; Wenjing Qiu; Li Fang; Rouyun Tan; Ping Wen; Junwei Yang Journal: Am J Physiol Renal Physiol Date: 2011-10-19
Authors: Pat Gulhati; Kanika A Bowen; Jianyu Liu; Payton D Stevens; Piotr G Rychahou; Min Chen; Eun Y Lee; Heidi L Weiss; Kathleen L O'Connor; Tianyan Gao; B Mark Evers Journal: Cancer Res Date: 2011-03-23 Impact factor: 12.701