Jiahong Chen1, Ilya A Shanin2,3, Shuwei Lv1, Qiang Wang4, Chuanbin Mao5, Zhenlin Xu1, Yuanming Sun1, Qing Wu1, Sergei A Eremin2,3, Hongtao Lei1. 1. Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Provincial Engineering and Technique Research Centre of Food Safety Detection and Risk Assessment, South China Agricultural University, Guangzhou, 510642, China. 2. Faculty of Chemistry, MV Lomonosov Moscow State University, 119991, Moscow, Russia. 3. AN Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia. 4. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China. 5. Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, OK, 73019, USA.
Abstract
BACKGROUND: Clinafloxacin is used for the treatment of disease in food-producing animals, e.g. Brucella melitensis, which often occurs in goats; however, the clinafloxacin residue in goat milk may harm human health and result in the development of drug-resistant bacterial strains or allergies. Despite this, there is not a rapid, sensitive and accurate analytical method in goat milk for rapid screening or monitoring purposes. RESULTS: One homologous and five heterologous tracers were designed and compared for fluorescence polarization immunoassay (FPIA) optimization. Based on the combination of a heterologous tracer (PAZ-FITC, synthesized with pazufloxacin and FITC) and the antibody against clinafloxacin, a highly sensitive FPIA was established for the detection of clinafloxacin residue in goat milk for the first time. The IC50 value was 29.3 µg L(-1) for clinafloxacin in the heterologous format - six times lower than that of the combination of the homologous tracers and the antibody. The recoveries ranged from 86.8% to 104.5%, with the relative standard deviation ranging from 4.1% to 7.2%. Validation by high-performance liquid chromatography (HPLC) confirmed that the results obtained from the proposed FPIA were in agreement with those of HPLC. CONCLUSION: This proposed heterologous strategy for enhanced FPIA is sensitive and rapid enough for the high-throughput detection of clinafloxacin residue in goat milk.
BACKGROUND:Clinafloxacin is used for the treatment of disease in food-producing animals, e.g. Brucella melitensis, which often occurs in goats; however, the clinafloxacin residue in goat milk may harm human health and result in the development of drug-resistant bacterial strains or allergies. Despite this, there is not a rapid, sensitive and accurate analytical method in goat milk for rapid screening or monitoring purposes. RESULTS: One homologous and five heterologous tracers were designed and compared for fluorescence polarization immunoassay (FPIA) optimization. Based on the combination of a heterologous tracer (PAZ-FITC, synthesized with pazufloxacin and FITC) and the antibody against clinafloxacin, a highly sensitive FPIA was established for the detection of clinafloxacin residue in goat milk for the first time. The IC50 value was 29.3 µg L(-1) for clinafloxacin in the heterologous format - six times lower than that of the combination of the homologous tracers and the antibody. The recoveries ranged from 86.8% to 104.5%, with the relative standard deviation ranging from 4.1% to 7.2%. Validation by high-performance liquid chromatography (HPLC) confirmed that the results obtained from the proposed FPIA were in agreement with those of HPLC. CONCLUSION: This proposed heterologous strategy for enhanced FPIA is sensitive and rapid enough for the high-throughput detection of clinafloxacin residue in goat milk.
Authors: Olga D Hendrickson; Nadezhda A Taranova; Anatoly V Zherdev; Boris B Dzantiev; Sergei A Eremin Journal: Sensors (Basel) Date: 2020-12-12 Impact factor: 3.576
Authors: Anna Raysyan; Robin Moerer; Bianca Coesfeld; Sergei A Eremin; Rudolf J Schneider Journal: Anal Bioanal Chem Date: 2020-11-25 Impact factor: 4.142