| Literature DB >> 2591372 |
D Bouchez1, J G Tokuhisa, D J Llewellyn, E S Dennis, J G Ellis.
Abstract
The ocs-element is an enhancer element first identified in the promoter of the octopine synthase gene (OCS) where it occurs as a 16 bp palindromic sequence. The transcriptional enhancing activity of the ocs-element correlated with in vitro binding of a transcription factor. We have now identified ocs-elements in the promoter regions of six other T-DNA genes involved in opine synthesis and three plant viral promoters including the 35S promoter of cauliflower mosaic virus. These elements bind the ocs transcription factor in vitro and enhance transcription in plant cells. Comparison of the sequences of these 10 elements has defined a 20 bp consensus sequence, TGACG(T/C)AAG(C/G)(G/A)(A/C)T(G/T)ACG(T/C)(A/C)(A/C), which includes the 16 bp palindrome in its central region. We propose the name ocs-element for this class of promoter elements of similar sequence and function.Entities:
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Year: 1989 PMID： 2591372 PMCID： PMC401614 DOI： 10.1002/j.1460-2075.1989.tb08605.x
Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598