C-J Hou1, Y-M Qi, D-Z Zhang, Q-G Wang, C-S Cui, L Kuang, B Chen. 1. Department of Congenital Heart Disease, Cardiovascular Research Institute of PLA, General Hospital of Shenyang Military Command, Shenyang, Liaoning Province, China. chuanjuhou@126.com.
Abstract
OBJECTIVE: This study aims to explore the effects of physical injury and stromal cell-derived factor-1α (SDF-1α) on the proliferation of cardiomyocytes and chemotactic effects of cardiomyocytes on the migration of cardiac fibroblasts. MATERIALS AND METHODS: Isolation and primary culture of rat cardiomyocytes and cardiac fibroblasts were performed; scratching was employed to induce physical injury on cells which were cultured with SDF-1α at different concentrations; proliferation ability of cardiomyocytes was checked with CCK-8 assay and migratory ability of cardiac fibroblasts under the chemotaxis of cardiomyocytes was detected with Transwell assay. RESULTS: SDF-1α enhanced the proliferation ability of cardiomyocytes with physical injury, especially at the concentration of 80 µg/L when the proliferation rate of cardiomyocytes increased most markedly. Moreover, physically injured cardiomyocyte that was cultured with SDF-1α significantly elevated migratory ability of cardiac fibroblasts, which tended to be more obvious along with the chemotactic culture time. CONCLUSIONS: SDF-1α enhanced the proliferation ability of cardiomyocytes with physical injury, and physically injured cardiomyocyte that was cultured with SDF-1α promoted the migration of cardiac fibroblasts.
OBJECTIVE: This study aims to explore the effects of physical injury and stromal cell-derived factor-1α (SDF-1α) on the proliferation of cardiomyocytes and chemotactic effects of cardiomyocytes on the migration of cardiac fibroblasts. MATERIALS AND METHODS: Isolation and primary culture of rat cardiomyocytes and cardiac fibroblasts were performed; scratching was employed to induce physical injury on cells which were cultured with SDF-1α at different concentrations; proliferation ability of cardiomyocytes was checked with CCK-8 assay and migratory ability of cardiac fibroblasts under the chemotaxis of cardiomyocytes was detected with Transwell assay. RESULTS: SDF-1α enhanced the proliferation ability of cardiomyocytes with physical injury, especially at the concentration of 80 µg/L when the proliferation rate of cardiomyocytes increased most markedly. Moreover, physically injured cardiomyocyte that was cultured with SDF-1α significantly elevated migratory ability of cardiac fibroblasts, which tended to be more obvious along with the chemotactic culture time. CONCLUSIONS: SDF-1α enhanced the proliferation ability of cardiomyocytes with physical injury, and physically injured cardiomyocyte that was cultured with SDF-1α promoted the migration of cardiac fibroblasts.
Authors: Dominika Lukovic; Katrin Zlabinger; Alfred Gugerell; Andreas Spannbauer; Noemi Pavo; Ljubica Mandic; Denise T Weidenauer; Stefan Kastl; Christoph Kaun; Aniko Posa; Inna Sabdyusheva Litschauer; Johannes Winkler; Mariann Gyöngyösi Journal: F1000Res Date: 2016-11-22