| Literature DB >> 25907042 |
Jennifer L Choi1, James-Kevin Y Tan1, Drew L Sellers2, Hua Wei1, Philip J Horner3, Suzie H Pun4.
Abstract
There is currently no cure for neuron loss in the brain, which can occur due to traumatic injury or neurodegenerative disease. One proposed method to enhanpan>ce brain neurogenesis is gene tranpan>sfer to neural progenitor cells. In this work, a pan> class="Chemical">guanidine-based copolymer was synthesized and compared to an amine-based copolymer analog previously shown to effectively deliver genes in the murine brain. The guanidine-based copolymer was more efficient at gene transfer to immortalized, cultured cell lines; however, the amine-based copolymer was more effective at gene transfer in the brain. DNA condensation studies revealed that the nucleic acid complexes formed with the guanidine-based copolymer were more susceptible to unpackaging in the presence of anionic proteoglycans compared to complexes formed with the amine-based copolymer. Therefore, polyplexes formed from the amine-based copolymer may be more resistant to destabilization by the heparan sulfate proteoglycans present in the stem cell niches of the brain.Entities:
Keywords: Brain; Gene delivery; Guanidine; Polymer
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Year: 2015 PMID: 25907042 PMCID: PMC4409668 DOI: 10.1016/j.biomaterials.2015.03.008
Source DB: PubMed Journal: Biomaterials ISSN: 0142-9612 Impact factor: 12.479