Zuhier Awan1, Maxime Denis2, Anna Roubtsova3, Rachid Essalmani3, Jadwiga Marcinkiewicz3, Amani Awan4, Hermann Gram5, Nabil G Seidah3, Jacques Genest6. 1. Division of Clinical Biochemistry, King Abdulaziz University, Jeddah, Saudi Arabia zawan@kau.edu.sa. 2. The Department Cardiology, McGill University, Montréal, Québec, Canada. 3. Institut de Recherches Cliniques de Montréal, Montréal, Québec, Canada. 4. Division of Clinical Biochemistry, King Abdulaziz University, Jeddah, Saudi Arabia. 5. Novartis Institutes for Biomedical Research, Basel, Switzerland. 6. The Department Cardiology, McGill University, Montréal, Québec, Canada jacques.genest@muhc.mcgill.ca.
Abstract
BACKGROUND: Given the link between cholesterol and activation of inflammation via interleukin 1β (IL-1β), we tested the effects of IL-1β inhibition on atherosclerotic calcification in mice. Patients with familial hypercholesterolemia develop extensive aortic calcification and calcific aortic stenosis. Although statins delay this process, low-density lipoprotein (LDL) cholesterol lowering alone is not enough to avert it. Data suggest that vascular inflammation initiated by hypercholesterolemia is followed by unchecked mineralization at sites of atherosclerotic plaques. The LDL-receptor (LDLR)-deficient (Ldlr(-/-)) and LDLR-attenuated Pcsk9(Tg) mice are available animal models for pharmacological testing. METHODS: A mouse monoclonal antibody (mAb) against IL-1β or placebo was administered subcutaneously in Ldlr(-/-) and Pcsk9(Tg) models fed a Western diet. Drug level, anthropometric, lipid, and glucose profiles were determined. Expressions of proprotein convertase subtilisin/kexin type 9 (PCSK9), serum amyloid A1, and cytokine were measured by enzyme-linked immunosorbent assay. Aortic calcification was determined by microcomputerized tomography (micro-CT) and X-ray densitometry, and aortic flow velocity was assessed by ultrasound. RESULTS: Circulating levels of IL-1β in Ldlr(-/-) mice were significantly greater (2-fold) than observed in Pcsk9(Tg) mice. Placebo- and mAb-treated mice did not differ in their growth, lipid, glucose profiles, and other cytokines. Calcifications were significantly diminished in mAb-treatment Ldlr(-/-) mice (a reduction of ∼ 75% by X-ray and ∼ 90% by micro-CT) and reduced insignificantly in mAb-treatment Pcsk9(Tg) mice, whereas aortic flow velocity was unchanged in both models. CONCLUSIONS: Herein, we demonstrate that aortic calcifications can be inhibited by an IL-1β mAb in LDLR-deficient mice. These results have a translational component to prevent vascular calcification in human and represent new evidence to rationalize targeting inflammation in cardiovascular disease.
BACKGROUND: Given the link between cholesterol and activation of inflammation via interleukin 1β (IL-1β), we tested the effects of IL-1β inhibition on atherosclerotic calcification in mice. Patients with familial hypercholesterolemia develop extensive aortic calcification and calcific aortic stenosis. Although statins delay this process, low-density lipoprotein (LDL) cholesterol lowering alone is not enough to avert it. Data suggest that vascular inflammation initiated by hypercholesterolemia is followed by unchecked mineralization at sites of atherosclerotic plaques. The LDL-receptor (LDLR)-deficient (Ldlr(-/-)) and LDLR-attenuated Pcsk9(Tg) mice are available animal models for pharmacological testing. METHODS: A mouse monoclonal antibody (mAb) against IL-1β or placebo was administered subcutaneously in Ldlr(-/-) and Pcsk9(Tg) models fed a Western diet. Drug level, anthropometric, lipid, and glucose profiles were determined. Expressions of proprotein convertase subtilisin/kexin type 9 (PCSK9), serum amyloid A1, and cytokine were measured by enzyme-linked immunosorbent assay. Aortic calcification was determined by microcomputerized tomography (micro-CT) and X-ray densitometry, and aortic flow velocity was assessed by ultrasound. RESULTS: Circulating levels of IL-1β in Ldlr(-/-) mice were significantly greater (2-fold) than observed in Pcsk9(Tg) mice. Placebo- and mAb-treated mice did not differ in their growth, lipid, glucose profiles, and other cytokines. Calcifications were significantly diminished in mAb-treatment Ldlr(-/-) mice (a reduction of ∼ 75% by X-ray and ∼ 90% by micro-CT) and reduced insignificantly in mAb-treatment Pcsk9(Tg) mice, whereas aortic flow velocity was unchanged in both models. CONCLUSIONS: Herein, we demonstrate that aortic calcifications can be inhibited by an IL-1β mAb in LDLR-deficient mice. These results have a translational component to prevent vascular calcification in human and represent new evidence to rationalize targeting inflammation in cardiovascular disease.
Authors: Alan A Sag; Adrian Covic; Gerard London; Marc Vervloet; David Goldsmith; Jose Luis Gorriz; Mehmet Kanbay Journal: Int Urol Nephrol Date: 2016-02-22 Impact factor: 2.370
Authors: Ahmed E Amer; George S G Shehatou; Hassan A El-Kashef; Manar A Nader; Ahmed R El-Sheakh Journal: Cardiovasc Drugs Ther Date: 2021-07-26 Impact factor: 3.727
Authors: Samantha J Borland; Julia Behnsen; Nick Ashton; Sheila E Francis; Keith Brennan; Michael J Sherratt; Philip J Withers; Ann E Canfield Journal: Int J Mol Sci Date: 2020-06-25 Impact factor: 5.923
Authors: Luca Liberale; Erik W Holy; Alexander Akhmedov; Nicole R Bonetti; Fabian Nietlispach; Christian M Matter; François Mach; Fabrizio Montecucco; Jürg H Beer; Francesco Paneni; Frank Ruschitzka; Peter Libby; Thomas F Lüscher; Giovanni G Camici Journal: J Clin Med Date: 2019-11-26 Impact factor: 4.241