Hakan Ak1, İsmail Gülşen2, Tamer Karaaslan3, İlker Alaca3, Aydın Candan4, Havva Koçak5, Tugay Atalay6, Asuman Çelikbilek7, İsmail Demir8, Tevfik Yılmaz7. 1. Department of Neurosurgery, Bozok University Faculty of Medicine, Yozgat, Turkey. nrsdrhakanak@yahoo.com. 2. Department of Neurosurgery, Yüzüncü Yıl University Faculty of Medicine, Van, Turkey. 3. Department of Neurosurgery, Süleyman Demirel University Faculty of Medicine, Isparta, Turkey. 4. Department of Histology and Embryology, Süleyman Demirel University Faculty of Medicine, Isparta, Turkey. 5. Department of Biocehmistry, Dumlupınar University Faculty of Medicine, Kütahya, Turkey. 6. Department of Neurosurgery, Bozok University Faculty of Medicine, Yozgat, Turkey. 7. Department of Neurosurgery, Dicle University Faculty of Medicine, Diyarbakır, Turkey. 8. Department of Neurosurgery, Van Regional Training and Teaching Hospital, Van, Turkey.
Abstract
BACKGROUND: The purpose of this study was to investigate the effects of Caffeic Acid Phenethyl Ester (CAPE) on proinflammatory cytokines, IL-1ß and TNF-α, and explore its healing effect after acute spinal cord injury. METHODS: Forty-eight male Wistar-Albino rats were used in this study which was planned as three groups. All groups were divided into two sub-groups. Group 1a was the control group, in which only lower segment thoracic laminectomy was performed. In group 1b, spinal cord trauma was performed with aneurysm clip. In the second group, serum physiologic was given systemically thirty minutes after trauma, and rats were sacrificed after the first and sixth hour. In the third group, CAPE was given systemically thirty minutes after trauma, and rats were sacrificed after the first and sixth hour. Serum IL-1ß and TNF-α levels were analyzed by ELISA in the serum. Histopathological analysis was performed in damaged cord tissues. RESULTS: CAPE suppressed TNF-α and IL-1ß levels in the serum. In histopathological evaluation, it was detected that CAPE decreased hemorrhage and necrosis. CONCLUSION: CAPE suppresses the levels of proinflammatory cytokines, TNF-α and IL-1ß, after acute spinal cord injury in the early phase and contributes to the healing process.
BACKGROUND: The purpose of this study was to investigate the effects of Caffeic Acid Phenethyl Ester (CAPE) on proinflammatory cytokines, IL-1ß and TNF-α, and explore its healing effect after acute spinal cord injury. METHODS: Forty-eight male Wistar-Albino rats were used in this study which was planned as three groups. All groups were divided into two sub-groups. Group 1a was the control group, in which only lower segment thoracic laminectomy was performed. In group 1b, spinal cord trauma was performed with aneurysm clip. In the second group, serum physiologic was given systemically thirty minutes after trauma, and rats were sacrificed after the first and sixth hour. In the third group, CAPE was given systemically thirty minutes after trauma, and rats were sacrificed after the first and sixth hour. Serum IL-1ß and TNF-α levels were analyzed by ELISA in the serum. Histopathological analysis was performed in damaged cord tissues. RESULTS:CAPE suppressed TNF-α and IL-1ß levels in the serum. In histopathological evaluation, it was detected that CAPE decreased hemorrhage and necrosis. CONCLUSION:CAPE suppresses the levels of proinflammatory cytokines, TNF-α and IL-1ß, after acute spinal cord injury in the early phase and contributes to the healing process.
Authors: Zheng Li; Xingye Li; Matthew T V Chan; William Ka Kei Wu; DunXian Tan; Jianxiong Shen Journal: J Cell Mol Med Date: 2017-04-21 Impact factor: 5.310