Literature DB >> 25904242

Comparison of nasopharyngeal aspirate with flocked swab for PCR-detection of respiratory viruses in children.

Hege Smith Tunsjø1, Are Stuitz Berg2, Christopher Steven Inchley2, Irmelin Kittelsen Røberg1, Truls Michael Leegaard3,4.   

Abstract

Fast- and high-throughput molecular workflows require sample matrices to be suitable for automation. Respiratory swabs are better suited for this purpose compared to the more viscous nasopharyngeal aspirates. Samples collected by nasopharyngeal aspiration and nasopharyngeal flocked swab from 81 children were compared for detection and recovery of respiratory viruses. Using real-time RT-PCR, no statistically significant differences in virus detection between the two sample types were found, supporting the use of flocked swabs in children aged one month to two years.
© 2015 APMIS. Published by John Wiley & Sons Ltd.

Entities:  

Keywords:  Aspirate; PCR; flocked swab; respiratory viruses

Mesh：

Year:  2015        PMID： 25904242     DOI： 10.1111/apm.12375

Source DB:  PubMed          Journal:  APMIS        ISSN： 0903-4641            Impact factor:   3.205


  7 in total

1.  Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections.

Authors:  Carmen L Charlton; Esther Babady; Christine C Ginocchio; Todd F Hatchette; Robert C Jerris; Yan Li; Mike Loeffelholz; Yvette S McCarter; Melissa B Miller; Susan Novak-Weekley; Audrey N Schuetz; Yi-Wei Tang; Ray Widen; Steven J Drews
Journal:  Clin Microbiol Rev       Date:  2018-12-12       Impact factor: 26.132

Review 2.  Understanding and utilizing textile-based electrostatic flocking for biomedical applications.

Authors:  Alec McCarthy; Rajesh Shah; Johnson V John; Demi Brown; Jingwei Xie
Journal:  Appl Phys Rev       Date:  2021-12       Impact factor: 19.162

3.  Asthma exacerbations in a subtropical area and the role of respiratory viruses: a cross-sectional study.

Authors:  Lusmaia Damaceno Camargo Costa; Paulo Augusto Moreira Camargos; Paul L P Brand; Fabíola Souza Fiaccadori; Menira Borges de Lima Dias E Souza; Divina das Dôres de Paula Cardoso; Ítalo de Araújo Castro; Ruth Minamisava; Paulo Sérgio Sucasas da Costa
Journal:  BMC Pulm Med       Date:  2018-07-03       Impact factor: 3.317

4.  Comparison of detection rate of 16 sampling methods for respiratory viruses: a Bayesian network meta-analysis of clinical data and systematic review.

Authors:  Nianzong Hou; Kai Wang; Haiyang Zhang; Mingjian Bai; Hao Chen; Weidong Song; Fusen Jia; Yi Zhang; Shiliang Han; Bing Xie
Journal:  BMJ Glob Health       Date:  2020-11

5.  Whole-Genome Shotgun Sequencing for Nasopharyngeal Microbiome in Pre-school Children With Recurrent Wheezing.

Authors:  Yuping Song; Jinpao Hou; Jamie Sui Lam Kwok; Haoyi Weng; Man Fung Tang; Maggie Haitian Wang; Agnes Sze Yin Leung; Kin Pong Tao; Gary Wing Kin Wong; Renee Wan Yi Chan; Stephen Kwok Wing Tsui; Ting Fan Leung
Journal:  Front Microbiol       Date:  2022-02-16       Impact factor: 5.640

6.  Assessment of perceived distress due to nasopharyngeal swab collection in healthy Indian infants participating in a clinical trial.

Authors:  Anand Kawade; Girish Dayma; Aditi Apte; Sudipto Roy; Arun Gondhali; Sanjay Juvekar; Ashish Bavdekar
Journal:  Paediatr Neonatal Pain       Date:  2021-12-26

7.  BCSH/BSBMT/UK clinical virology network guideline: diagnosis and management of common respiratory viral infections in patients undergoing treatment for haematological malignancies or stem cell transplantation.

Authors:  Fiona L Dignan; Andrew Clark; Celia Aitken; Maria Gilleece; Vishal Jayakar; Pramila Krishnamurthy; Antonio Pagliuca; Michael N Potter; Bronwen Shaw; Roderick Skinner; Andrew Turner; Robert F Wynn; Peter Coyle
Journal:  Br J Haematol       Date:  2016-04-07       Impact factor: 6.998
  7 in total

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