OBJECTIVE: Optimized production and quality control of gallium-68 labeled ethylenediamine tetramethylene phosphonate ((68)Ga-EDTMP) as an efficient PET radiotracer for bone scans have been presented. METHODS: Efforts have been made to present a fast, efficient, cost-effective and facile protocol for (68)Ga-EDTMP productions for clinical trials. (68)Ga-EDTMP was prepared using generator-based (68)GaCl3 and EDTMP at optimized conditions for time, temperature, ligand amount, gallium content followed by proper formulation. The biodistribution of the tracer in rats was studied using tissue counting and PET/CT imaging up to 155 min. RESULTS: (68)Ga-EDTMP was prepared at optimized conditions in 5-10 min at 50-60 °C (radiochemical purity ≈99 ± 0.88 % ITLC, >99 % HPLC, specific activity: 15-18 GBq/mM). The biodistribution of the tracer demonstrated high bone uptake of the tracer in 10-20 min while yielding the best images in 2 h. CONCLUSION: The whole production and quality control of (68)Ga-EDTMP including labeling, purification, HPLC analysis, sterilization and LAL test took 18-20 min with significant specific activity for administration to limited number of patients in a PET center.
OBJECTIVE: Optimized production and quality control of gallium-68 labeled ethylenediamine tetramethylene phosphonate ((68)Ga-EDTMP) as an efficient PET radiotracer for bone scans have been presented. METHODS: Efforts have been made to present a fast, efficient, cost-effective and facile protocol for (68)Ga-EDTMP productions for clinical trials. (68)Ga-EDTMP was prepared using generator-based (68)GaCl3 and EDTMP at optimized conditions for time, temperature, ligand amount, gallium content followed by proper formulation. The biodistribution of the tracer in rats was studied using tissue counting and PET/CT imaging up to 155 min. RESULTS: (68)Ga-EDTMP was prepared at optimized conditions in 5-10 min at 50-60 °C (radiochemical purity ≈99 ± 0.88 % ITLC, >99 % HPLC, specific activity: 15-18 GBq/mM). The biodistribution of the tracer demonstrated high bone uptake of the tracer in 10-20 min while yielding the best images in 2 h. CONCLUSION: The whole production and quality control of (68)Ga-EDTMP including labeling, purification, HPLC analysis, sterilization and LAL test took 18-20 min with significant specific activity for administration to limited number of patients in a PET center.
Authors: Douglas S MacPherson; Kimberly Fung; Brendon E Cook; Lynn C Francesconi; Brian M Zeglis Journal: Dalton Trans Date: 2019-10-07 Impact factor: 4.390
Authors: Tahereh Firuzyar; Amir Reza Jalilian; Mohammad Reza Aboudzadeh; Hossein Sadeghpour; Mahdi Shafiee-Ardestani; Ali Khalaj Journal: Indian J Nucl Med Date: 2016 Oct-Dec
Authors: René Fernández; Elisabeth Eppard; Wencke Lehnert; Luis David Jiménez-Franco; Cristian Soza-Ried; Matías Ceballos; Jessica Ribbeck; Andreas Kluge; Frank Rösch; Marian Meckel; Konstantin Zhernosekov; Vasko Kramer; Horacio Amaral Journal: J Nucl Med Date: 2021-01-08 Impact factor: 10.057