Xun Yang1, Bei-Bei Chen1, Ming-Hua Zhang1, Xin-Rong Wang2. 1. Respiratory Department, Central Hospital of Wuhan, Wuhan 430014, China. 2. Respiratory Department, Central Hospital of Wuhan, Wuhan 430014, China. Electronic address: 1844209009@qq.com.
Abstract
OBJECTIVE: To study the role of microRNA-126 in the development of lung cancer. METHODS: The biological function of microRNA-126 was detected using EdU assay and CCK-8 assay; the target gene of microRNA-126 was analyzed using real time RT-PCR and Western blot assay. RESULTS: In A549 cell line, overexpression of microRNA-126 inhibits the proliferation rate; VEGF is the target gene of microRNA-126; microRNA-126 exerts its function via regulating VEGF protein level. CONCLUSIONS: microRNA-126 inhibits the proliferation in A549 cell line.
OBJECTIVE: To study the role of microRNA-126 in the development of lung cancer. METHODS: The biological function of microRNA-126 was detected using EdU assay and CCK-8 assay; the target gene of microRNA-126 was analyzed using real time RT-PCR and Western blot assay. RESULTS: In A549 cell line, overexpression of microRNA-126 inhibits the proliferation rate; VEGF is the target gene of microRNA-126; microRNA-126 exerts its function via regulating VEGF protein level. CONCLUSIONS: microRNA-126 inhibits the proliferation in A549 cell line.