Literature DB >> 25902070

Correction: Regulation of MYC Expression and Differential JQ1 Sensitivity in Cancer Cells.

Trent Fowler, Payel Ghatak, David H Price, Ronald Conaway, Joan Conaway, Cheng-Ming Chiang, James E Bradner, Ali Shilatifard, Ananda L Roy.   

Abstract

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Year:  2015        PMID: 25902070      PMCID: PMC4406619          DOI: 10.1371/journal.pone.0126328

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


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There is an error in Fig 2 that appears to have occurred during the preparation of files after the manuscript was accepted. In Fig 2B, the blot for Brd4 incorrectly appears as a duplicate of P-Brd4. The authors have provided a corrected version of Fig 2 here.
Fig 2

Brd4 occupancy and expression in different cells.

Cells were either untreated or treated with 1 μM of JQ1 for 2 hours. (A) Chromatin Immunoprecipitation (ChIP) across MYC with anti-C-terminal Brd4 antibody. Each experiment was performed twice, analyzed in triplicate via real-time PCR and reported as the mean and standard deviation of the two experiments. A representation of the promoter area of MYC is provided for orientation. (B) Western blotting to detect (far left) Brd4 (~180 KD) and (middle) Brd4-S484/488-phos (P-Brd4, ~220 KD) was performed three times. A non-specific band detected with phopsho-Brd4 antibody is denoted with an asterisk. Typical results are shown with densitometry analysis relative to CREB expression, which is used as a normalization control (far right).

Brd4 occupancy and expression in different cells.

Cells were either untreated or treated with 1 μM of JQ1 for 2 hours. (A) Chromatin Immunoprecipitation (ChIP) across MYC with anti-C-terminal Brd4 antibody. Each experiment was performed twice, analyzed in triplicate via real-time PCR and reported as the mean and standard deviation of the two experiments. A representation of the promoter area of MYC is provided for orientation. (B) Western blotting to detect (far left) Brd4 (~180 KD) and (middle) Brd4-S484/488-phos (P-Brd4, ~220 KD) was performed three times. A non-specific band detected with phopsho-Brd4 antibody is denoted with an asterisk. Typical results are shown with densitometry analysis relative to CREB expression, which is used as a normalization control (far right).
  1 in total

1.  Regulation of MYC expression and differential JQ1 sensitivity in cancer cells.

Authors:  Trent Fowler; Payel Ghatak; David H Price; Ronald Conaway; Joan Conaway; Cheng-Ming Chiang; James E Bradner; Ali Shilatifard; Ananda L Roy
Journal:  PLoS One       Date:  2014-01-23       Impact factor: 3.240

  1 in total

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