| Literature DB >> 25902070 |
Trent Fowler, Payel Ghatak, David H Price, Ronald Conaway, Joan Conaway, Cheng-Ming Chiang, James E Bradner, Ali Shilatifard, Ananda L Roy.
Abstract
Entities:
Year: 2015 PMID: 25902070 PMCID: PMC4406619 DOI: 10.1371/journal.pone.0126328
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 2Brd4 occupancy and expression in different cells.
Cells were either untreated or treated with 1 μM of JQ1 for 2 hours. (A) Chromatin Immunoprecipitation (ChIP) across MYC with anti-C-terminal Brd4 antibody. Each experiment was performed twice, analyzed in triplicate via real-time PCR and reported as the mean and standard deviation of the two experiments. A representation of the promoter area of MYC is provided for orientation. (B) Western blotting to detect (far left) Brd4 (~180 KD) and (middle) Brd4-S484/488-phos (P-Brd4, ~220 KD) was performed three times. A non-specific band detected with phopsho-Brd4 antibody is denoted with an asterisk. Typical results are shown with densitometry analysis relative to CREB expression, which is used as a normalization control (far right).