Xing Zhong1, Yijun Du1, Yuan Lei2, Nina Liu1, Yanyun Guo1, Tianrong Pan3. 1. Department of Endocrinology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230061, PR China. 2. Department of Endocrinology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China. 3. Department of Endocrinology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230061, PR China. Electronic address: pantianrong1968@163.com.
Abstract
PURPOSE: The purpose of this study was to investigate the association of VDR polymorphism with development of retinopathy in a Han Chinese population with type 2 diabetes mellitus. MATERIALS AND METHODS: A total of 204 T2DM patients were subdivided into groups without diabetic retinopathy (NDR, n=110) and those with DR (n=94). VDR rs2228570 (FokI:C>T), rs1544410 (BsmI:G>A), and rs7975232 (Apal:A>C) polymorphism was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: Diabetes duration (10.0 vs. 5.0 years, P<0.01) was longer, systolic blood pressure (143.98 ± 24.31 vs. 135.11 ± 15.23, P<0.01), and HbA1c (9.2 ± 2.06 vs. 8.35 ± 1.62, P<0.01) were higher in DR than in NDR patients. Distribution frequencies of the rs2228570, rs1544410, and rs7975232 genotypes followed the Hardy-Weinberg equilibrium. VDR rs2228570 TT genotype frequency was significantly higher in DR (n=30; 31.9%) than in NDR patients (n=14; 12.7%; P<0.01). DR patients carried more rs2228570 T alleles (n=113; 60.1%) than did NDR patients (n=89; 40.5%; P<0.01). Genotype frequencies of rs1544410 and rs7975232 in NDR and DR patients were not different. Logistic analysis confirmed that diabetes duration (odds ratio (OR) 1.108, P<0.01), SBP (OR 1.022, P<0.05), HbA1c (OR 1.267, P<0.05), and the VDR rs2228570 T allele (OR 1.467, P<0.01) were independently associated with DR risk. TAA haplotype frequency was significantly higher in DR (24.0%) than in NDR (16.1%) patients (P<0.05). CONCLUSIONS: Diabetes duration, SBP, HbA1c, and the rs2228570 T allele were associated with increased risk of DR. VDR rs2228570 might be good candidate biomarker of DR in Han Chinese T2DM patients.
PURPOSE: The purpose of this study was to investigate the association of VDR polymorphism with development of retinopathy in a Han Chinese population with type 2 diabetes mellitus. MATERIALS AND METHODS: A total of 204 T2DM patients were subdivided into groups without diabetic retinopathy (NDR, n=110) and those with DR (n=94). VDRrs2228570 (FokI:C>T), rs1544410 (BsmI:G>A), and rs7975232 (Apal:A>C) polymorphism was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS:Diabetes duration (10.0 vs. 5.0 years, P<0.01) was longer, systolic blood pressure (143.98 ± 24.31 vs. 135.11 ± 15.23, P<0.01), and HbA1c (9.2 ± 2.06 vs. 8.35 ± 1.62, P<0.01) were higher in DR than in NDRpatients. Distribution frequencies of the rs2228570, rs1544410, and rs7975232 genotypes followed the Hardy-Weinberg equilibrium. VDRrs2228570 TT genotype frequency was significantly higher in DR (n=30; 31.9%) than in NDRpatients (n=14; 12.7%; P<0.01). DR patients carried more rs2228570 T alleles (n=113; 60.1%) than did NDRpatients (n=89; 40.5%; P<0.01). Genotype frequencies of rs1544410 and rs7975232 in NDR and DR patients were not different. Logistic analysis confirmed that diabetes duration (odds ratio (OR) 1.108, P<0.01), SBP (OR 1.022, P<0.05), HbA1c (OR 1.267, P<0.05), and the VDRrs2228570 T allele (OR 1.467, P<0.01) were independently associated with DR risk. TAA haplotype frequency was significantly higher in DR (24.0%) than in NDR (16.1%) patients (P<0.05). CONCLUSIONS:Diabetes duration, SBP, HbA1c, and the rs2228570 T allele were associated with increased risk of DR. VDRrs2228570 might be good candidate biomarker of DR in Han Chinese T2DM patients.
Authors: Alicia J Jenkins; Mugdha V Joglekar; Anandwardhan A Hardikar; Anthony C Keech; David N O'Neal; Andrzej S Januszewski Journal: Rev Diabet Stud Date: 2015-08-10