Rutger Matthes1,2, Lukasz Jablonowski3,4, Ina Koban3, Antje Quade4, Nils-Olaf Hübner5, Rabea Schlueter6, Klaus-Dieter Weltmann4, Thomas von Woedtke4, Axel Kramer5, Thomas Kocher3. 1. Unit of Periodontology, Dental School, University of Greifswald, Rotgerberstr. 8, 17475, Greifswald, Germany. rutger.matthes@uni-greifswald.de. 2. Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany. rutger.matthes@uni-greifswald.de. 3. Unit of Periodontology, Dental School, University of Greifswald, Rotgerberstr. 8, 17475, Greifswald, Germany. 4. Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany. 5. Institute of Hygiene and Environmental Medicine, University Medicine Greifswald, Walther-Rathenau-Str. 49 a, 17475, Greifswald, Germany. 6. Institute of Microbiology, University of Greifswald, Friedrich-Ludwig-Jahn-Str. 15, 17487, Greifswald, Germany.
Abstract
OBJECTIVES: To prevent oral candidiasis, it is crucial to inactivate Candida-based biofilms on dentures. Common denture cleansing solutions cannot sufficiently inactivate Candida albicans. Therefore, we investigated the anticandidal efficacy of a physical plasma against C. albicans biofilms in vitro. MATERIALS AND METHODS: Argon or argon plasma with 1 % oxygen admixture was applied on C. albicans biofilms grown for 2, 7, or 16 days on polymethylmethacrylate discs; 0.1 % chlorhexidine digluconate (CHX) and 0.6 % sodium hypochlorite (NaOCl) solutions served as positive treatment controls. In addition, these two solutions were applied in combination with plasma for 30 min to assess potential synergistic effects. The anticandidal efficacy was determined by the number of colony forming units (CFU) in log(10) and expressed as reduction factor (RF, the difference between control and treated specimen). RESULTS: On 2-day-biofilms, plasma treatment alone or combined with 30 min CHX treatment led to significant differences of means of CFU (RF = 4.2 and RF = 4.3), clearly superior to CHX treatment alone (RF = 0.6). Plasma treatment of 7-day-or 16-day-old biofilms revealed no significant CFU reduction. The treatment of 7-day-old (RF = 1.7) and 16-day-old (RF = 1.3) biofilms was slightly more effective with NaOCl alone than with the combined treatment of NaOCl and plasma (RF = 1.6/RF = 1.9). The combination of CHX and plasma increased the RF immaterially. CONCLUSION: The use of plasma alone and in combination with antiseptics is promising anticandidal regimens for daily use on dentures when biofilms are not older than 2 days. CLINICAL RELEVANCE: Plasma could help to reduce denture-associated candidiasis.
OBJECTIVES: To prevent oral candidiasis, it is crucial to inactivate Candida-based biofilms on dentures. Common denture cleansing solutions cannot sufficiently inactivate Candida albicans. Therefore, we investigated the anticandidal efficacy of a physical plasma against C. albicans biofilms in vitro. MATERIALS AND METHODS:Argon or argon plasma with 1 % oxygen admixture was applied on C. albicans biofilms grown for 2, 7, or 16 days on polymethylmethacrylate discs; 0.1 % chlorhexidine digluconate (CHX) and 0.6 % sodium hypochlorite (NaOCl) solutions served as positive treatment controls. In addition, these two solutions were applied in combination with plasma for 30 min to assess potential synergistic effects. The anticandidal efficacy was determined by the number of colony forming units (CFU) in log(10) and expressed as reduction factor (RF, the difference between control and treated specimen). RESULTS: On 2-day-biofilms, plasma treatment alone or combined with 30 min CHX treatment led to significant differences of means of CFU (RF = 4.2 and RF = 4.3), clearly superior to CHX treatment alone (RF = 0.6). Plasma treatment of 7-day-or 16-day-old biofilms revealed no significant CFU reduction. The treatment of 7-day-old (RF = 1.7) and 16-day-old (RF = 1.3) biofilms was slightly more effective with NaOCl alone than with the combined treatment of NaOCl and plasma (RF = 1.6/RF = 1.9). The combination of CHX and plasma increased the RF immaterially. CONCLUSION: The use of plasma alone and in combination with antiseptics is promising anticandidal regimens for daily use on dentures when biofilms are not older than 2 days. CLINICAL RELEVANCE: Plasma could help to reduce denture-associated candidiasis.
Authors: Rafał Brożek; Ryszard Koczorowski; Rafał Rogalewicz; Adam Voelkel; Beata Czarnecka; John W Nicholson Journal: Dent Mater Date: 2010-12-08 Impact factor: 5.304
Authors: Rutger Matthes; Claudia Bender; Rabea Schlüter; Ina Koban; René Bussiahn; Stephan Reuter; Jürgen Lademann; Klaus-Dieter Weltmann; Axel Kramer Journal: PLoS One Date: 2013-07-24 Impact factor: 3.240
Authors: Judith Napp; Georg Daeschlein; Matthias Napp; Sebastian von Podewils; Denis Gümbel; Romy Spitzmueller; Paolo Fornaciari; Peter Hinz; Michael Jünger Journal: GMS Hyg Infect Control Date: 2015-06-02