Literature DB >> 25897611

Monoclonal antibody against M protein of spring viremia of carp virus.

Zeming Li1, Qi Zhang, Peixiao Luo, Guangxin Liu, Min Wang, Xueqin Liu.   

Abstract

M protein of spring viremia of carp virus (SVCV) was expressed in Escherichia coli and then used to immunize BALB/c mice. One monoclonal antibody (5A1) against M protein was generated by fusion of mouse myeloma cell line SP2/0 and spleen lymphocytes from immunized mice. The characterizations of this MAb were confirmed by ELISA (enzyme linked immunosorbent assay), IFA (immunofluorescent assay), and Western blot analysis. All results indicate that MAb 5A1 was specific to SVCV M protein.

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Year:  2015        PMID： 25897611      PMCID： PMC4410279          DOI： 10.1089/mab.2014.0056

Source DB:  PubMed          Journal:  Monoclon Antib Immunodiagn Immunother        ISSN： 2167-9436


  11 in total

1.  Determination of the complete genomic sequence and analysis of the gene products of the virus of Spring Viremia of Carp, a fish rhabdovirus.

Authors:  Bernd Hoffmann; Heike Schütze; Thomas C Mettenleiter
Journal:  Virus Res       Date:  2002-03-20       Impact factor: 3.303

2.  Interaction between tubulin and the viral matrix protein of vesicular stomatitis virus: possible implications in the viral cytopathic effect.

Authors:  R Melki; Y Gaudin; D Blondel
Journal:  Virology       Date:  1994-07       Impact factor: 3.616

3.  Monoclonal antibodies against NS3 and NS5 proteins of Japanese encephalitis virus.

Authors:  Zheng Chen; Lin Shao; Jing Ye; Yongmao Li; Shaomei Huang; Huanchun Chen; Shengbo Cao
Journal:  Hybridoma (Larchmt)       Date:  2012-04

4.  Phylogenetic analysis of spring viraemia of carp virus isolates from Austria indicates the existence of at least two subgroups within genogroup Id.

Authors:  A Basic; O Schachner; I Bilic; M Hess
Journal:  Dis Aquat Organ       Date:  2009-05-27       Impact factor: 1.802

5.  Inhibition of host transcription by vesicular stomatitis virus involves a novel mechanism that is independent of phosphorylation of TATA-binding protein (TBP) or association of TBP with TBP-associated factor subunits.

Authors:  H Yuan; S Puckett; D S Lyles
Journal:  J Virol       Date:  2001-05       Impact factor: 5.103

6.  Monoclonal antibodies against NS4B protein of japanese encephalitis virus.

Authors:  Xindi Ruan; Shaomei Huang; Lin Shao; Jing Ye; Zheng Chen; Huanchun Chen; Shengbo Cao
Journal:  Monoclon Antib Immunodiagn Immunother       Date:  2013-12

7.  Identification of spring viraemia of carp virus (SVCV) by combined RT-PCR and nested PCR.

Authors:  M Koutná; T Veselý; I Psikal; J Hůlová
Journal:  Dis Aquat Organ       Date:  2003-08-04       Impact factor: 1.802

8.  The cell-rounding activity of the vesicular stomatitis virus matrix protein is due to the induction of cell death.

Authors:  Sarah A Kopecky; Douglas S Lyles
Journal:  J Virol       Date:  2003-05       Impact factor: 5.103

9.  Development and evaluation of a one-step loop-mediated isothermal amplification for detection of spring viraemia of carp virus.

Authors:  Z Liu; Y Teng; X Xie; H Li; J Lv; L Gao; F Tian; Y Jiang; Z Chu; C Xie; H Liu
Journal:  J Appl Microbiol       Date:  2008-07-10       Impact factor: 3.772

10.  Molecular analysis of spring viraemia of carp virus in China: a fatal aquatic viral disease that might spread in East Asian.

Authors:  Nian Zhi Zhang; Li Feng Zhang; Yi Nan Jiang; Ting Zhang; Chun Xia
Journal:  PLoS One       Date:  2009-07-22       Impact factor: 3.240
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