Literature DB >> 25897609

Generation and Characterization of a New Monoclonal Antibody Against CXCL4.

Jing Gao1, Mingyuan Wu, Jin Gao, Xia Wang, Yang Zhang, Shunying Zhu, Yan Yu, Wei Han.   

Abstract

CXCL4 plays important roles in numerous disease processes, which makes the CXCL4 signaling pathway a potential therapeutic target. In this study, we aimed to develop a neutralizing antibody against both human and mouse CXCL4. Rats were immunized with recombinant human CXCL4 (rhCXCL4). Hybridoma clones were created by fusion of the immunized rat spleen cells with mouse myeloma SP2/0 cells and screened using recombinant mouse CXCL4 (rmCXCL4) and rhCXCL4. The CXCL4 monoclonal antibody (CXCL4 MAb) produced by the 16D6-3 hybridoma clone was sequenced and characterized by Western blot and Biacore assays. It recognized both human and mouse CXCL4 with high affinity and neutralized the effect of rhCXCL4 in vitro. Thus, the antibody may be used in the studies of CXCL4 in murine disease models and as a template in the antibody humanization for clinical developments.

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Year:  2015        PMID： 25897609      PMCID： PMC4410284          DOI： 10.1089/mab.2014.0050

Source DB:  PubMed          Journal:  Monoclon Antib Immunodiagn Immunother        ISSN： 2167-9436


  34 in total

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Journal:  Blood       Date:  1990-03-15       Impact factor: 22.113

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Journal:  Science       Date:  1980-08-01       Impact factor: 47.728

5.  Platelet factor 4 promotes adhesion of hematopoietic progenitor cells and binds IL-8: novel mechanisms for modulation of hematopoiesis.

Authors:  Arkadiusz Z Dudek; Irina Nesmelova; Kevin Mayo; Catherine M Verfaillie; Simon Pitchford; Arne Slungaard
Journal:  Blood       Date:  2003-02-13       Impact factor: 22.113

6.  Activation of p38-MAPK by CXCL4/CXCR3 axis contributes to p53-dependent intestinal apoptosis initiated by 5-fluorouracil.

Authors:  Jing Gao; Jin Gao; Lan Qian; Xia Wang; Mingyuan Wu; Yang Zhang; Hao Ye; Shunying Zhu; Yan Yu; Wei Han
Journal:  Cancer Biol Ther       Date:  2014-05-06       Impact factor: 4.742

7.  Platelet factor 4 in conjunction with IL-4 directs differentiation of human monocytes into specialized antigen-presenting cells.

Authors:  Ingo Fricke; Daniell Mitchell; Frank Petersen; Andreas Böhle; Silvia Bulfone-Paus; Sven Brandau
Journal:  FASEB J       Date:  2004-08-19       Impact factor: 5.191

8.  Platelet factor 4/CXCL4 induces phagocytosis and the generation of reactive oxygen metabolites in mononuclear phagocytes independently of Gi protein activation or intracellular calcium transients.

Authors:  Olga Pervushina; Barbara Scheuerer; Norbert Reiling; Lars Behnke; Jens-M Schröder; Brigitte Kasper; Ernst Brandt; Silvia Bulfone-Paus; Frank Petersen
Journal:  J Immunol       Date:  2004-08-01       Impact factor: 5.422

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Authors:  T F Deuel; P S Keim; M Farmer; R L Heinrikson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

10.  An alternatively spliced variant of CXCR3 mediates the inhibition of endothelial cell growth induced by IP-10, Mig, and I-TAC, and acts as functional receptor for platelet factor 4.

Authors:  Laura Lasagni; Michela Francalanci; Francesco Annunziato; Elena Lazzeri; Stefano Giannini; Lorenzo Cosmi; Costanza Sagrinati; Benedetta Mazzinghi; Claudio Orlando; Enrico Maggi; Fabio Marra; Sergio Romagnani; Mario Serio; Paola Romagnani
Journal:  J Exp Med       Date:  2003-06-02       Impact factor: 14.307
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  1 in total

Review 1.  Monoclonal antibodies: Principles and applications of immmunodiagnosis and immunotherapy for hepatitis C virus.

Authors:  Ashraf Tabll; Aymn T Abbas; Sherif El-Kafrawy; Ahmed Wahid
Journal:  World J Hepatol       Date:  2015-10-08
  1 in total

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