| Literature DB >> 25896758 |
Wataru Shoji1, Yusuke Suenaga2, Yoshiki Kaneko3, S M Rafiqul Islam3, Jennifer Alagu3, Sana Yokoi4, Masaki Nio5, Akira Nakagawara6.
Abstract
NCYM is a cis-antisense gene of MYCN and is amplified in human neuroblastomas. High NCYM expression is associated with poor prognoses, and the NCYM protein stabilizes MYCN to promote proliferation of neuroblastoma cells. However, the molecular mechanisms of NCYM in the regulation of cell survival have remained poorly characterized. Here we show that NCYM promotes cleavage of MYCN to produce the anti-apoptotic protein, Myc-nick, both in vitro and in vivo. NCYM and Myc-nick were induced at G2/M phase, and NCYM knockdown induced apoptotic cell death accompanied by Myc-nick downregulation. These results reveal a novel function of NCYM as a regulator of Myc-nick production in human neuroblastomas.Entities:
Keywords: Calpain; Cell cycle; MYCN; Myc-nick; NCYM; Neuroblastoma
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Year: 2015 PMID： 25896758 DOI： 10.1016/j.bbrc.2015.04.050
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575