Wenjing Zheng1, Zhen Liu2, Wei Zhang3, Xiaoxia Hu4. 1. Department of Gynecology, Renmin Hospital of Wuhan University, Zhang Zhidong Road, Wuhan, 430060, Hubei, China. zwj4949@163.com. 2. Department of Gynecology, Renmin Hospital of Wuhan University, Zhang Zhidong Road, Wuhan, 430060, Hubei, China. 3. Department of Gynecology, Renmin Hospital of Wuhan University, Zhang Zhidong Road, Wuhan, 430060, Hubei, China. zw6676@163.com. 4. Department of Gynecology, The People Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, Guanxi, China. huxxia@hotmail.com.
Abstract
BACKGROUND: MicroRNAs are frequently altered in numerous cancers and are critical regulators of various diseases. miR-31 has been shown to be significantly altered in a variety of cancers. METHODS: In the present study, we measured the expression level of miR-31 in cervical cancer, CIN and normal cervical tissues by real-time RT (reverse transcription)-PCR. We also analyzed the correlations between the expression level of miR-31 and the clinical characteristics in cases of cervical squamous cell carcinoma. In addition, we measured the expression of miR-31 in cervical cancer cell lines, and transfected HPV16 E6 siRNA and HPV16 E7 siRNA into SiHa cells to investigate the effects on miR-31. Finally, the effects of miR-31 on cell proliferation, migration and invasion were measured in HeLa and SiHa cells that were transfected with a miR-31 mimic or a negative control. RESULT: We found that the expression level of miR-31 was significantly higher in cervical cancer patients than in normal individuals (P < 0.05). Aberrant expression of miR-31 was positively correlated with the lymph node metastasis (LNM), vessel invasion and HPV status (P < 0.05). Additionally, miR-31 was also overexpressed in the cervical cancer-derived HeLa and SiHa cells compared with C33A cells (P < 0.05). Moreover, a relationship was found between miR-31 expression and the HPV16 oncoproteins E6/E7. Furthermore, we found that the overexpression of miR-31 can promote cell proliferation and enhance the migration and invasion abilities of cancer cells. CONCLUSIONS: Our results suggested that miR-31 plays an oncogenetic role in the development and progression of cervical cancer.
BACKGROUND: MicroRNAs are frequently altered in numerous cancers and are critical regulators of various diseases. miR-31 has been shown to be significantly altered in a variety of cancers. METHODS: In the present study, we measured the expression level of miR-31 in cervical cancer, CIN and normal cervical tissues by real-time RT (reverse transcription)-PCR. We also analyzed the correlations between the expression level of miR-31 and the clinical characteristics in cases of cervical squamous cell carcinoma. In addition, we measured the expression of miR-31 in cervical cancer cell lines, and transfected HPV16 E6 siRNA and HPV16 E7 siRNA into SiHa cells to investigate the effects on miR-31. Finally, the effects of miR-31 on cell proliferation, migration and invasion were measured in HeLa and SiHa cells that were transfected with a miR-31 mimic or a negative control. RESULT: We found that the expression level of miR-31 was significantly higher in cervical cancerpatients than in normal individuals (P < 0.05). Aberrant expression of miR-31 was positively correlated with the lymph node metastasis (LNM), vessel invasion and HPV status (P < 0.05). Additionally, miR-31 was also overexpressed in the cervical cancer-derived HeLa and SiHa cells compared with C33A cells (P < 0.05). Moreover, a relationship was found between miR-31 expression and the HPV16 oncoproteins E6/E7. Furthermore, we found that the overexpression of miR-31 can promote cell proliferation and enhance the migration and invasion abilities of cancer cells. CONCLUSIONS: Our results suggested that miR-31 plays an oncogenetic role in the development and progression of cervical cancer.
Authors: Elgar Susanne Quabius; Immanuel Merz; Tibor Görögh; Jürgen Hedderich; Jochen Haag; Christoph Röcken; Petra Ambrosch; Markus Hoffmann Journal: Papillomavirus Res Date: 2017-06-16
Authors: Barbara Pardini; Daniela De Maria; Antonio Francavilla; Cornelia Di Gaetano; Guglielmo Ronco; Alessio Naccarati Journal: BMC Cancer Date: 2018-06-27 Impact factor: 4.430
Authors: Laura Izquierdo; Ruth Montalbo; Mercedes Ingelmo-Torres; Carme Mallofré; Miguel Ramírez-Backhaus; Jose Rubio; Antoine G Van der Heijden; Ewout Schaafsma; Antonio Lopez-Beltran; Ana Blanca; Nathan Lawrentschuk; Antonio Alcaraz; Lourdes Mengual Journal: Oncotarget Date: 2017-05-16
Authors: Jing Fan Ma; Peng Fei Wei; Chang Guo; Yuan Peng Shi; Yang Lv; Long Xin Qiu; Long Ping Wen Journal: Biomed Res Int Date: 2018-05-24 Impact factor: 3.411