| Literature DB >> 25893780 |
Fuguang Li1, Guangyi Fan2, Cairui Lu1, Guanghui Xiao3, Changsong Zou1, Russell J Kohel4, Zhiying Ma5, Haihong Shang1, Xiongfeng Ma1, Jianyong Wu1, Xinming Liang2, Gai Huang3, Richard G Percy4, Kun Liu1, Weihua Yang1, Wenbin Chen2, Xiongming Du1, Chengcheng Shi2, Youlu Yuan1, Wuwei Ye1, Xin Liu2, Xueyan Zhang1, Weiqing Liu2, Hengling Wei1, Shoujun Wei1, Guodong Huang2, Xianlong Zhang6, Shuijin Zhu7, He Zhang2, Fengming Sun2, Xingfen Wang5, Jie Liang2, Jiahao Wang2, Qiang He2, Leihuan Huang2, Jun Wang2, Jinjie Cui1, Guoli Song1, Kunbo Wang1, Xun Xu2, John Z Yu4, Yuxian Zhu3, Shuxun Yu1.
Abstract
Gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (AtDt) genome. Here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map. In our assembly 88.5% of the 2,173-Mb scaffolds, which cover 89.6%∼96.7% of the AtDt genome, are anchored and oriented to 26 pseudochromosomes. Comparison of this G. hirsutum AtDt genome with the already sequenced diploid Gossypium arboreum (AA) and Gossypium raimondii (DD) genomes revealed conserved gene order. Repeated sequences account for 67.2% of the AtDt genome, and transposable elements (TEs) originating from Dt seem more active than from At. Reduction in the AtDt genome size occurred after allopolyploidization. The A or At genome may have undergone positive selection for fiber traits. Concerted evolution of different regulatory mechanisms for Cellulose synthase (CesA) and 1-Aminocyclopropane-1-carboxylic acid oxidase1 and 3 (ACO1,3) may be important for enhanced fiber production in G. hirsutum.Entities:
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Year: 2015 PMID： 25893780 DOI： 10.1038/nbt.3208
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908