| Literature DB >> 25892023 |
B Álvarez1, Z Escalona1, H Uenishi2, D Toki3, C Revilla1, M Yuste1, M Gómez Del Moral4, F Alonso1, A Ezquerra1, J Domínguez5.
Abstract
A cDNA clone encoding a 380 a-a type 1 transmembrane protein with homology to human Siglec-3/CD33 was obtained from a swine small intestine library. An analysis of protein sequence identified two immunoglobulin-like domains, a transmembrane region, and a carboxi-terminal tail with two tyrosine-based signalling motifs. Binding assays of Siglec-3 transfected CHO cells to polyacrylamide glycoconjugates showed a preference for α2-6-linked sialic acids. Using mAbs raised against a fragment containing the two Ig-like domains, porcine Siglec-3 was found to be expressed on monocytes and granulocytes, and their bone marrow precursors. It was also detected in lymph node, splenic and alveolar macrophages. MAbs immunoprecipitated, from granulocyte lysates, a protein of 51-60 kDa under both non-reducing and reducing conditions. MAbs were also used to analyse functional activity of Siglec-3 on bone marrow and blood cells. Engagement of Siglec-3 by mAb had no apparent effect on cell proliferation or cytokine production.Entities:
Keywords: CD33; Monoclonal antibody; Monocyte; Myeloid cells; Siglec-3; Swine
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Year: 2015 PMID： 25892023 DOI： 10.1016/j.dci.2015.04.002
Source DB: PubMed Journal: Dev Comp Immunol ISSN： 0145-305X Impact factor: 3.636