| Literature DB >> 25885569 |
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Abstract
Entities:
Year: 2015 PMID: 25885569 PMCID: PMC4401760 DOI: 10.1371/journal.pone.0126197
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 2Molecular signatures created by the insertion of IS26.
A: The 3´-CS of class 1 integrons in pASL01a and pAKU_1 has been structurally modified by different IS26-mediated deletion events such that PCR with L1 and JL-D2 primers is expected to generate 2082 and 2342 bp long amplicons respectively. B: Eight base pair signature sequences created by the insertion of IS26 found flanking the inverted repeats of IS26 elements clearly suggest that the CRL in strain 2011EC-3493 is a derivative of Tn6029C while 2009–2050 is a derivative of Tn6029B described previously in pHCM1. The eight base repeat on the left end of Tn6029B and that in 2009EL-2050 are different because in pHCM1 (Tn6029B) there is a deletion of the class 1 integron whereas in 2009EL-2050 the fragment adjacent to the left hand end of the insertion site is inverted by IS26-mediated events. These signatures are also consistent with the proposition that Tn6029C evolved from Tn6029B.