Paula Renata Lima Machado1, Kleber Juvenal Silva Farias1, Maira Gabriela Martins Pereira2, Patrícia Pereira da Silva de Freitas3, Benedito Antônio Lopes da Fonseca4. 1. PhD. Biomedical Scientist, Virology Research Center, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil. 2. MSc. Biologist, Virology Research Center, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil. 3. PhD. Veterinarian, Virology Research Center, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil. 4. MD, PhD. Associate Professor, Virology Research Center, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
Abstract
CONTEXT AND OBJECTIVE: Kaposi's sarcoma (KS) is a common neoplastic disease in AIDS patients. The aim of this study was to evaluate the frequency of human herpesvirus 8 (HHV-8) infection in human immunodeficiency virus (HIV)-infected patients, with or without KS manifestations and correlate HHV-8 detection with KS staging. DESIGN AND SETTING: Analytic cross-sectional study conducted in a public tertiary-level university hospital in Ribeirão Preto, São Paulo, Brazil. METHODS: Antibodies against HHV-8 lytic-phase antigens were detected by means of the immunofluorescence assay. HHV-8 DNA was detected in the patient samples through a nested polymerase chain reaction (nested PCR) that amplified a region of open reading frame (ORF)-26 of HHV-8. RESULTS: Anti-HHV-8 antibodies were detected in 30% of non-KS patients and 100% of patients with KS. Furthermore, the HHV-8 DNA detection rates observed in HIV-positive patients with KS were 42.8% in serum, 95.4% in blood samples and 100% in skin biopsies; and in patients without KS, the detection rate was 4% in serum. Out of the 16 serum samples from patients with KS-AIDS who were classified as stage II, two were positive (12.5%); and out of the 33 samples from patients in stage IV, 19 (57.6%) were positive. CONCLUSION: We observed an association between HHV-8 detection and disease staging, which was higher in the serum of patients in stage IV. This suggests that detection of HHV-8 DNA in serum could be very useful for clinical assessment of patients with KS and for monitoring disease progression.
CONTEXT AND OBJECTIVE:Kaposi's sarcoma (KS) is a common neoplastic disease in AIDSpatients. The aim of this study was to evaluate the frequency of human herpesvirus 8 (HHV-8) infection in human immunodeficiency virus (HIV)-infectedpatients, with or without KS manifestations and correlate HHV-8 detection with KS staging. DESIGN AND SETTING: Analytic cross-sectional study conducted in a public tertiary-level university hospital in Ribeirão Preto, São Paulo, Brazil. METHODS: Antibodies against HHV-8 lytic-phase antigens were detected by means of the immunofluorescence assay. HHV-8 DNA was detected in the patient samples through a nested polymerase chain reaction (nested PCR) that amplified a region of open reading frame (ORF)-26 of HHV-8. RESULTS: Anti-HHV-8 antibodies were detected in 30% of non-KS patients and 100% of patients with KS. Furthermore, the HHV-8 DNA detection rates observed in HIV-positivepatients with KS were 42.8% in serum, 95.4% in blood samples and 100% in skin biopsies; and in patients without KS, the detection rate was 4% in serum. Out of the 16 serum samples from patients with KS-AIDS who were classified as stage II, two were positive (12.5%); and out of the 33 samples from patients in stage IV, 19 (57.6%) were positive. CONCLUSION: We observed an association between HHV-8 detection and disease staging, which was higher in the serum of patients in stage IV. This suggests that detection of HHV-8 DNA in serum could be very useful for clinical assessment of patients with KS and for monitoring disease progression.
Authors: Nakougou Moϊ-Bohm Biatougou; Muriel S Ouedraogo; Serge Theophile Soubeiga; Theodora Mahoukede Zohoncon; Paul Ouedraogo; Dorcas Obiri-Yeboah; Aziz Sidi Aristide Tapsoba; Touwendpoulimdé Isabelle Kiendrebeogo; Tani Sagna; Pascal Niamba; Adama Traore; Jacques Simpore Journal: HIV AIDS (Auckl) Date: 2022-07-08
Authors: Haruna Muwonge; Hassan Kasujja; Nixon Niyonzima; Carolyne Atugonza; Josephine Kasolo; Allan Lugaajju; Joshua Nfambi; Sembajwe Larry Fred; Ali Moses Damani; Ivan Kimuli; Robert Zavuga; Faith Nakazzi; Edgar Kigozi; Damalie Nakanjako; David Patrick Kateete; Freddie Bwanga Journal: Noncoding RNA Res Date: 2022-04-10
Authors: Cathy Logan; Kathryn Todorof; Suzanne P Fiorillo; Thomas B Campbell; John H Elder; Margaret Borok; Ivy Gudza; Lovemore Gwanzura; Buxton Ndemera; Michael J Lochhead; Constance A Benson; Robert T Schooley Journal: PLoS One Date: 2016-09-26 Impact factor: 3.240