Arlene E Dent1, Rie Nakajima2, Li Liang2, Elisabeth Baum2, Ann M Moormann3, Peter Odada Sumba4, John Vulule4, Denise Babineau5, Arlo Randall6, D Huw Davies2, Philip L Felgner2, James W Kazura7. 1. Center for Global Health and Diseases, Case Western Reserve University Rainbow Babies and Children's Hospital, Cleveland, Ohio. 2. University of California, Irvine. 3. Center for Global Health Research, University of Massachusetts Medical School, Worcester. 4. Kenya Medical Research Institute, Kisumu. 5. Rho Inc, Chapel Hill, North Carolina. 6. Antigen Discovery, Inc., Irvine, California. 7. Center for Global Health and Diseases, Case Western Reserve University.
Abstract
BACKGROUND: Immunoglobulin G antibodies (Abs) to Plasmodium falciparum antigens have been associated with naturally acquired immunity to symptomatic malaria. METHODS: We probed protein microarrays covering 824 unique P. falciparum protein features with plasma from residents of a community in Kenya monitored for 12 weeks for (re)infection and symptomatic malaria after administration of antimalarial drugs. P. falciparum proteins recognized by Abs from 88 children (aged 1-14 years) and 86 adults (aged ≥ 18 years), measured at the beginning of the observation period, were ranked by Ab signal intensity. RESULTS: Abs from immune adults reacted with a total 163 of 824 P. falciparum proteins. Children gradually acquired Abs to the full repertoire of antigens recognized by adults. Abs to some antigens showed high seroconversion rates, reaching maximal levels early in childhood, whereas others did not reach adult levels until adolescence. No correlation between Ab signal intensity and time to (re)infection was observed. In contrast, Ab levels to 106 antigens were significantly higher in children who were protected from symptomatic malaria compared with those who were not. Abs to antigens predictive of protection included P. falciparum erythrocyte membrane protein 1, merozoite surface protein (MSP) 10, MSP2, liver-stage antigen 3, PF70, MSP7, and Plasmodium helical interspersed subtelomeric domain protein. CONCLUSIONS: Protein microarrays may be useful in the search for malaria antigens associated with protective immunity.
BACKGROUND: Immunoglobulin G antibodies (Abs) to Plasmodium falciparum antigens have been associated with naturally acquired immunity to symptomatic malaria. METHODS: We probed protein microarrays covering 824 unique P. falciparum protein features with plasma from residents of a community in Kenya monitored for 12 weeks for (re)infection and symptomatic malaria after administration of antimalarial drugs. P. falciparum proteins recognized by Abs from 88 children (aged 1-14 years) and 86 adults (aged ≥ 18 years), measured at the beginning of the observation period, were ranked by Ab signal intensity. RESULTS: Abs from immune adults reacted with a total 163 of 824 P. falciparum proteins. Children gradually acquired Abs to the full repertoire of antigens recognized by adults. Abs to some antigens showed high seroconversion rates, reaching maximal levels early in childhood, whereas others did not reach adult levels until adolescence. No correlation between Ab signal intensity and time to (re)infection was observed. In contrast, Ab levels to 106 antigens were significantly higher in children who were protected from symptomatic malaria compared with those who were not. Abs to antigens predictive of protection included P. falciparum erythrocyte membrane protein 1, merozoite surface protein (MSP) 10, MSP2, liver-stage antigen 3, PF70, MSP7, and Plasmodium helical interspersed subtelomeric domain protein. CONCLUSIONS: Protein microarrays may be useful in the search for malaria antigens associated with protective immunity.
Authors: Jelle J Goeman; Jan Oosting; Anne-Marie Cleton-Jansen; Jakob K Anninga; Hans C van Houwelingen Journal: Bioinformatics Date: 2005-01-18 Impact factor: 6.937
Authors: C J Drakeley; P H Corran; P G Coleman; J E Tongren; S L R McDonald; I Carneiro; R Malima; J Lusingu; A Manjurano; W M M Nkya; M M Lemnge; J Cox; H Reyburn; E M Riley Journal: Proc Natl Acad Sci U S A Date: 2005-03-25 Impact factor: 11.205
Authors: Blaise Genton; Inoni Betuela; Ingrid Felger; Fadwa Al-Yaman; Robin F Anders; Allan Saul; Lawrence Rare; Moses Baisor; Kerry Lorry; Graham V Brown; David Pye; David O Irving; Thomas A Smith; Hans-Peter Beck; Michael P Alpers Journal: J Infect Dis Date: 2002-02-14 Impact factor: 5.226
Authors: P Daubersies; A W Thomas; P Millet; K Brahimi; J A Langermans; B Ollomo; L BenMohamed; B Slierendregt; W Eling; A Van Belkum; G Dubreuil; J F Meis; C Guérin-Marchand; S Cayphas; J Cohen; H Gras-Masse; P Druilhe; L B Mohamed Journal: Nat Med Date: 2000-11 Impact factor: 53.440
Authors: Alice S Tarun; Xinxia Peng; Ronald F Dumpit; Yuko Ogata; Hilda Silva-Rivera; Nelly Camargo; Thomas M Daly; Lawrence W Bergman; Stefan H I Kappe Journal: Proc Natl Acad Sci U S A Date: 2008-01-02 Impact factor: 11.205
Authors: Laurence Florens; Michael P Washburn; J Dale Raine; Robert M Anthony; Munira Grainger; J David Haynes; J Kathleen Moch; Nemone Muster; John B Sacci; David L Tabb; Adam A Witney; Dirk Wolters; Yimin Wu; Malcolm J Gardner; Anthony A Holder; Robert E Sinden; John R Yates; Daniel J Carucci Journal: Nature Date: 2002-10-03 Impact factor: 49.962
Authors: Jo-Anne Chan; Danielle I Stanisic; Michael F Duffy; Leanne J Robinson; Enmoore Lin; James W Kazura; Christopher L King; Peter M Siba; Freya Ji Fowkes; Ivo Mueller; James G Beeson Journal: Eur J Immunol Date: 2017-09-13 Impact factor: 5.532
Authors: Thomas Jaenisch; Kirsten Heiss; Nico Fischer; Carolin Geiger; F Ralf Bischoff; Gerhard Moldenhauer; Leszek Rychlewski; Ali Sié; Boubacar Coulibaly; Peter H Seeberger; Lucjan S Wyrwicz; Frank Breitling; Felix F Loeffler Journal: Mol Cell Proteomics Date: 2019-01-10 Impact factor: 5.911
Authors: Fiona J McCallum; Kristina E M Persson; Freya J I Fowkes; Linda Reiling; Cleopatra K Mugyenyi; Jack S Richards; Julie A Simpson; Thomas N Williams; Paul R Gilson; Anthony N Hodder; Paul R Sanders; Robin F Anders; David L Narum; Chetan Chitnis; Brendan S Crabb; Kevin Marsh; James G Beeson Journal: J Leukoc Biol Date: 2016-11-11 Impact factor: 4.962
Authors: Vincent van Unen; Mikhael D Manurung; Koen A Stam; Sanne E de Jong; Jelle J Goeman; Simon P Jochems; Thomas Höllt; Nicola Pezzotti; Yoanne D Mouwenda; Madeleine Eunice Betouke Ongwe; Freia-Raphaella Lorenz; Yvonne C M Kruize; Shohreh Azimi; Marion H König; Anna Vilanova; Elmar Eisemann; Boudewijn P F Lelieveldt; Meta Roestenberg; B Kim Lee Sim; Marcel J T Reinders; Rolf Fendel; Stephen L Hoffman; Peter G Kremsner; Frits Koning; Benjamin Mordmüller; Bertrand Lell; Maria Yazdanbakhsh Journal: Nat Immunol Date: 2021-04-22 Impact factor: 25.606
Authors: Sandra K Nilsson Bark; Rushdy Ahmad; Kathleen Dantzler; Amanda K Lukens; Mariana De Niz; Matthew J Szucs; Xiaoying Jin; Joanne Cotton; Dietmar Hoffmann; Eva Bric-Furlong; Ray Oomen; Mark Parrington; Dan Milner; Daniel E Neafsey; Steven A Carr; Dyann F Wirth; Matthias Marti Journal: Mol Cell Proteomics Date: 2017-11-21 Impact factor: 5.911
Authors: Aarti Jain; Omid Taghavian; Derek Vallejo; Emmanuel Dotsey; Dan Schwartz; Florian G Bell; Chad Greef; D Huw Davies; Jennipher Grudzien; Abraham P Lee; Philip L Felgner; Li Liang Journal: Proteomics Date: 2016-03-29 Impact factor: 3.984