M Yang1, H Yuan, M Miao, W Xu. 1. Department of Orthopedics, Changhai Hospital, the Second Military Medical University, 200433, Shanghai, P. R. China.
Abstract
BACKGROUND: The goal of the present study was to investigate the osteogenic potential of ligament fibroblasts from patients with ankylosing spondylitis (AS). MATERIALS AND METHODS: Ligament fibroblasts were isolated from tissues harvested from AS patients and patients with aseptic necrosis of the femoral head (ANFH) who had undergone total hip arthroplasty (THA). Cells were cultured in osteogenic induction medium (OIM) with or without bone morphogenetic protein 2 (BMP-2) for 30 days. During this time, the levels of alkaline phosphatase (ALP) and osteocalcin (OC) were determined as markers of osteogenesis. RESULTS: Ligament fibroblasts isolated from patients with AS exhibited significantly greater potential for differentiation than those isolated from patients with osteoarthritis (ANFH). CONCLUSION: These findings illuminate osteogenesis as a new pathway for studying the mechanism underlying ankylosis in AS patients. Factors which cause localized stromal remodeling at the enthesis significantly influence formation of new bone and further research is required to determine the mechanisms responsible for the osteogenic potential of enthesis cells and ligament fibroblasts in AS patients.
BACKGROUND: The goal of the present study was to investigate the osteogenic potential of ligament fibroblasts from patients with ankylosing spondylitis (AS). MATERIALS AND METHODS: Ligament fibroblasts were isolated from tissues harvested from AS patients and patients with aseptic necrosis of the femoral head (ANFH) who had undergone total hip arthroplasty (THA). Cells were cultured in osteogenic induction medium (OIM) with or without bone morphogenetic protein 2 (BMP-2) for 30 days. During this time, the levels of alkaline phosphatase (ALP) and osteocalcin (OC) were determined as markers of osteogenesis. RESULTS: Ligament fibroblasts isolated from patients with AS exhibited significantly greater potential for differentiation than those isolated from patients with osteoarthritis (ANFH). CONCLUSION: These findings illuminate osteogenesis as a new pathway for studying the mechanism underlying ankylosis in AS patients. Factors which cause localized stromal remodeling at the enthesis significantly influence formation of new bone and further research is required to determine the mechanisms responsible for the osteogenic potential of enthesis cells and ligament fibroblasts in AS patients.
Authors: D A Binks; E M Gravallese; D Bergin; R J Hodgson; A L Tan; M M Matzelle; D McGonagle; A Radjenovic Journal: Ann Rheum Dis Date: 2013-10-04 Impact factor: 19.103
Authors: Line Dam Heftdal; Thomas Andersen; Ditte Jæhger; Anders Woetmann; René Østgård; Elisabeth E Kenngott; Uta Syrbe; Joachim Sieper; Malene Hvid; Bent Deleuran; Tue W Kragstrup Journal: J Mol Med (Berl) Date: 2017-04-02 Impact factor: 4.599
Authors: Cong Ma; Bo Wen; Qin Zhang; Pei-Pei Shao; Wen Gu; Kun Qu; Yang Shi; Bei Wang Journal: Drug Des Devel Ther Date: 2019-02-11 Impact factor: 4.162