Maryam Alsadat Hashemipour1, Amir Reza Ghasemi2, Mahdi Ansari Dogaheh3, Molok Torabi4. 1. Department of Oral Medicine, Kerman Oral and Dental Disease Research Center, Kerman University of Medical Sciences, Kerman, Iran; email: m_hashemipoor@kmu.ac.ir. 2. Kerman Oral and Dental Disease Research Center, Kerman University of Medical Sciences, Kerman, Iran. 3. Department of Pharmaceutics, Kerman University of Medical Sciences, Kerman, Iran. 4. Department of Oral Pathology, Kerman Oral and Dental Disease Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Abstract
UNLABELLED: Introduction. Ulcers are one of the most frequent diseases af- fecting the oral cavity. The aim of this study was to assess the effects of omega-3 fatty acid (topical and systemic) on oral mucosa wound healing in rats. METHODS AND MATERIALS: In this study, adult male rats in 5 groups (n = 16 per group) were used (L: local, S: systemic, LB: local blank, SB: systemic blank, and C: control.) A wound (2 mm di- ameter) was punched into the hard palate of each rat. A mucosal de- fect (2 mm in diameter and 0.2 mm in wall thickness) was made to the depth of the periosteum in the palate with a round stainless steel blade designed for punch biopsy. For topical application, a swab was soaked in L omega-3 or LB and packed into the wound. For systemic treatment, 2 cc of the S omega-3 acid or SB was guided into the stom- ach by gastric gavage. The control group was not treated. Histological samples were harvested on post-injury days 2, 4, 6, and 8. RESULTS: The highest amount of polymorphonuclear (PMN) cells was observed on days 2 and 4 in the LB group. The control group had the highest inflammation score on days 2 and 4, and the lowest reepithelialization score on days 2, 4, 6, and 8. The thickest epithelium was observed in the L and S groups on days 6 and 8. CONCLUSION: Omega-3 fatty acid (L and S) increases fibroblast counts and decreases PMN cell counts. Moreover, this compound causes an increase in reepithelialization and epithelial thickness. .
UNLABELLED: Introduction. Ulcers are one of the most frequent diseases af- fecting the oral cavity. The aim of this study was to assess the effects of omega-3 fatty acid (topical and systemic) on oral mucosa wound healing in rats. METHODS AND MATERIALS: In this study, adult male rats in 5 groups (n = 16 per group) were used (L: local, S: systemic, LB: local blank, SB: systemic blank, and C: control.) A wound (2 mm di- ameter) was punched into the hard palate of each rat. A mucosal de- fect (2 mm in diameter and 0.2 mm in wall thickness) was made to the depth of the periosteum in the palate with a round stainless steel blade designed for punch biopsy. For topical application, a swab was soaked in L omega-3 or LB and packed into the wound. For systemic treatment, 2 cc of the S omega-3 acid or SB was guided into the stom- ach by gastric gavage. The control group was not treated. Histological samples were harvested on post-injury days 2, 4, 6, and 8. RESULTS: The highest amount of polymorphonuclear (PMN) cells was observed on days 2 and 4 in the LB group. The control group had the highest inflammation score on days 2 and 4, and the lowest reepithelialization score on days 2, 4, 6, and 8. The thickest epithelium was observed in the L and S groups on days 6 and 8. CONCLUSION:Omega-3 fatty acid (L and S) increases fibroblast counts and decreases PMN cell counts. Moreover, this compound causes an increase in reepithelialization and epithelial thickness. .
Authors: Paulo Goberlânio de Barros Silva; Érian Bezerra Belo de Codes; Milena Oliveira Freitas; Joyce Ohana de Lima Martins; Ana Paula Negreiros Nunes Alves; Fabrício Bitu Sousa Journal: J Oral Maxillofac Pathol Date: 2018 Sep-Dec