Literature DB >> 2587211

The ubiquitous nuclear protein, NHP1, binds with high affinity to different sequences of the chicken vitellogenin II gene.

M J Hughes1, J P Jost.   

Abstract

In gel shift assays, affinity chromatography-purified NHP1 forms a stable complex with different sequences of the chicken vitellogenin II gene. The apparent KD of NHP1 with the estrogen response element (ERE) containing 5-methylcytosine is 1 X 10(-11) M. NHP1 does not form a complex with the Xenopus vitellogenin ERE where the GCG bases are replaced by CAG. NHP1 is closely related if not identical to the other ubiquitous proteins NHP2, NHP3 and NHP4 that bind specifically to different sequences. All four proteins behave identically on chromatography and give identical patterns in proteolytic bandshift assays. NHP1, NHP2 and NHP3 have a native molecular weight of 170,000 and are composed of two polypeptides of 85 and 75 kDa. The possible function of NHP1 is discussed.

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Year:  1989        PMID： 2587211      PMCID： PMC335023          DOI： 10.1093/nar/17.21.8511

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  Genomic sequencing reveals a positive correlation between the kinetics of strand-specific DNA demethylation of the overlapping estradiol/glucocorticoid-receptor binding sites and the rate of avian vitellogenin mRNA synthesis.

Authors:  H P Saluz; J Jiricny; J P Jost
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

2.  Affinity purification of sequence-specific DNA binding proteins.

Authors:  J T Kadonaga; R Tjian
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

3.  An estrogen-responsive element derived from the 5' flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells.

Authors:  L Klein-Hitpass; M Schorpp; U Wagner; G U Ryffel
Journal:  Cell       Date:  1986-09-26       Impact factor: 41.582

4.  Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.

Authors:  A Seiler-Tuyns; P Walker; E Martinez; A M Mérillat; F Givel; W Wahli
Journal:  Nucleic Acids Res       Date:  1986-11-25       Impact factor: 16.971

5.  Chromatin structural transitions and the phenomenon of vitellogenin gene memory in chickens.

Authors:  J B Burch; M I Evans
Journal:  Mol Cell Biol       Date:  1986-06       Impact factor: 4.272

6.  Evolution of vitellogenin genes: comparative analysis of the nucleotide sequences downstream of the transcription initiation site of four Xenopus laevis and one chicken gene.

Authors:  J E Germond; P Walker; B ten Heggeler; M Brown-Luedi; E de Bony; W Wahli
Journal:  Nucleic Acids Res       Date:  1984-11-26       Impact factor: 16.971

7.  The C. elegans vitellogenin genes: short sequence repeats in the promoter regions and homology to the vertebrate genes.

Authors:  J Spieth; K Denison; S Kirtland; J Cane; T Blumenthal
Journal:  Nucleic Acids Res       Date:  1985-07-25       Impact factor: 16.971

8.  Structure and sequence of the promoter area and of a 5' upstream demethylation site of the estrogen-regulated chicken vitellogenin ii gene.

Authors:  M Geiser; I W Mattaj; A F Wilks; M Seldran; J P Jost
Journal:  J Biol Chem       Date:  1983-07-25       Impact factor: 5.157

9.  Octamer transcription factors bind to two different sequence motifs of the immunoglobulin heavy chain promoter.

Authors:  I Kemler; E Schreiber; M M Müller; P Matthias; W Schaffner
Journal:  EMBO J       Date:  1989-07       Impact factor: 11.598

10.  Association of transcriptionally active vitellogenin II gene with the nuclear matrix of chicken liver.

Authors:  J P Jost; M Seldran
Journal:  EMBO J       Date:  1984-09       Impact factor: 11.598
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  2 in total

1.  Estradiol down regulates the binding activity of an avian vitellogenin gene repressor (MDBP-2) and triggers a gradual demethylation of the mCpG pair of its DNA binding site.

Authors:  J P Jost; H P Saluz; A Pawlak
Journal:  Nucleic Acids Res       Date:  1991-10-25       Impact factor: 16.971

2.  Positive and negative regulatory elements of chicken vitellogenin II gene characterized by in vitro transcription competition assays in a homologous system.

Authors:  M Vaccaro; A Pawlak; J P Jost
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205
  2 in total

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