Peer W Kämmerer1, Michael Lehnert2,3, Bilal Al-Nawas4, Vinay V Kumar5, Sebastien Hagmann6, Abdulmonem Alshihri7, Bernhard Frerich1, Michael Veith2. 1. Department of Oral, Maxillofacial, and Plastic Surgery, University Medical Center Rostock, Rostock, Germany. 2. Laboratory of Biophysics, Westphalian University of Applied Sciences, Campus Recklinghausen, Recklinghausen, Germany. 3. Department of Microsystems Engineering - IMTEK University of Freiburg, Mainz, Germany. 4. Department of Oral, Maxillofacial, and Plastic Surgery, University Medical Center Mainz, Mainz, Germany. 5. Department of Oral and Maxillofacial Surgery, M R Ambedkar Dental College & Hospital 1/36, Karnataka, India. 6. Department of Orthopedics, University of Heidelberg, Heidelberg, Germany. 7. Department of Restorative and Biomaterial Sciences, Harvard School of Dental Medicine, Boston, MA, USA.
Abstract
BACKGROUND: Biofunctionalized implant surfaces may accelerate bony integration and increase long-term stability. PURPOSE: The aim of the study was to evaluate the osseous reaction toward biomimetic titanium implants surfaces coated with quasicovalent immobilized fibronectin in an in vivo animal model. MATERIALS AND METHODS: A total of 84 implants (uncoated [control 1, n = 36], streptavidin-biotin coated [test 1, n = 24], streptavidin-biotin-fibronectin coated [test 2, n = 24]) were inserted 1 mm supracortically in the proximal tibia of 12 rabbits. The samples were examined after 3 and 6 weeks. Total bone-implant contact (tBIC; %), bone-implant contact in the cortical (cBIC; %) and in the spongious bone (sBIC; %) as well as the percentage of linear bone fill (PLF; %) were evaluated. RESULTS: After 3 weeks, streptavidin-biotin-fibronectin implants had a significant higher sBIC (p = .043) and PLF (p = .007) compared with the uncoated samples. After 6 weeks, this difference was significant for tBIC (p = .016) and cBIC (p < .001). Additionally, uncoated screws showed a significant higher sBIC when compared with the fibronectin coating (p < .001). Streptavidin-biotin-coated implants showed less bone growth at both time points of all examined parameters when compared with their counterparts (all p < .001). CONCLUSIONS: Quasicovalent immobilization of biotinylated fibronectin with the streptavidin-biotin-fibronectin system on smooth surface titanium shows a beneficial faster osseous healing in vivo. Besides, an antifouling effect of the streptavidin-biotin coating was proven.
BACKGROUND: Biofunctionalized implant surfaces may accelerate bony integration and increase long-term stability. PURPOSE: The aim of the study was to evaluate the osseous reaction toward biomimetic titanium implants surfaces coated with quasicovalent immobilized fibronectin in an in vivo animal model. MATERIALS AND METHODS: A total of 84 implants (uncoated [control 1, n = 36], streptavidin-biotin coated [test 1, n = 24], streptavidin-biotin-fibronectin coated [test 2, n = 24]) were inserted 1 mm supracortically in the proximal tibia of 12 rabbits. The samples were examined after 3 and 6 weeks. Total bone-implant contact (tBIC; %), bone-implant contact in the cortical (cBIC; %) and in the spongious bone (sBIC; %) as well as the percentage of linear bone fill (PLF; %) were evaluated. RESULTS: After 3 weeks, streptavidin-biotin-fibronectin implants had a significant higher sBIC (p = .043) and PLF (p = .007) compared with the uncoated samples. After 6 weeks, this difference was significant for tBIC (p = .016) and cBIC (p < .001). Additionally, uncoated screws showed a significant higher sBIC when compared with the fibronectin coating (p < .001). Streptavidin-biotin-coated implants showed less bone growth at both time points of all examined parameters when compared with their counterparts (all p < .001). CONCLUSIONS: Quasicovalent immobilization of biotinylated fibronectin with the streptavidin-biotin-fibronectin system on smooth surface titanium shows a beneficial faster osseous healing in vivo. Besides, an antifouling effect of the streptavidin-biotin coating was proven.
Authors: Andreas Pabst; Ashraf Asran; Steffen Lüers; Markus Laub; Christopher Holfeld; Victor Palarie; Daniel G E Thiem; Philipp Becker; Amely Hartmann; Diana Heimes; Bilal Al-Nawas; Peer W Kämmerer Journal: Biomedicines Date: 2022-04-19
Authors: Oscar G Bodelón; Celia Clemente; Miguel Angel Alobera; Soledad Aguado-Henche; María Lorenza Escudero; María Cristina García Alonso Journal: Int J Implant Dent Date: 2016-07-21
Authors: Peer W Kämmerer; Malte Scholz; Maria Baudisch; Jan Liese; Katharina Wegner; Bernhard Frerich; Hermann Lang Journal: Stem Cells Int Date: 2017-08-16 Impact factor: 5.443