Literature DB >> 25870071

Evaluation of a modified EUCAST fragmented-mycelium inoculum method for in vitro susceptibility testing of dermatophytes and the activity of novel antifungal agents.

B Risslegger1, C Lass-Flörl1, G Blum1, M Lackner2.   

Abstract

For antifungal susceptibility testing of nonsporulating or poorly sporulating dermatophytes, a fragmented-mycelium inoculum preparation method was established and compared to broth microdilution testing according to CLSI and EUCAST guidelines. Moreover, the in vitro activity of new antifungal agents against dermatophytes was evaluated. Agreement between the mycelial inoculum method and the CLSI broth microdilution method was high (93% to 100%). Echinocandins (minimal effective concentration [MEC], ≤0.5 mg/liter) and posaconazole (MIC, ≤3.00 mg/liter) showed good activity against all tested dermatophytes.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Entities:  

Mesh：

Substances：

Year:  2015        PMID： 25870071      PMCID： PMC4432114          DOI： 10.1128/AAC.04381-14

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  7 in total

1.  [New apects in the diagnosis and therapy of dermatomycoses].

Authors:  J Brasch
Journal:  Hautarzt       Date:  2012-05       Impact factor: 0.751

2.  EUCAST Technical Note on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia-forming moulds.

Authors: 
Journal:  Clin Microbiol Infect       Date:  2008-10       Impact factor: 8.067

3.  Long-term effectiveness of treatment with terbinafine vs itraconazole in onychomycosis: a 5-year blinded prospective follow-up study.

Authors:  Bárour Sigurgeirsson; Jón H Olafsson; Jón B Steinsson; Carle Paul; Stephan Billstein; E Glyn V Evans
Journal:  Arch Dermatol       Date:  2002-03

4.  Standardization of a hyphal inoculum of aspergilli for amphotericin B susceptibility testing.

Authors:  V Bezjak
Journal:  J Clin Microbiol       Date:  1985-04       Impact factor: 5.948

5.  The Fragmentation of the Mycelium of Penicillium notatum and Penicillium chrysogenum by a High-Speed Blender and the Evaluation of Blended Seed.

Authors:  G M Savage; M J Brook
Journal:  J Bacteriol       Date:  1946-09       Impact factor: 3.490

6.  Susceptibility screening of hyphae-forming fungi with a new, easy, and fast inoculum preparation method.

Authors:  Arno Schmalreck; Birgit Willinger; Viktor Czaika; Wolfgang Fegeler; Karsten Becker; Gerhard Blum; Cornelia Lass-Flörl
Journal:  Mycopathologia       Date:  2012-08-04       Impact factor: 2.574

Review 7.  A review of the new antifungals: posaconazole, micafungin, and anidulafungin.

Authors:  Noah Scheinfeld
Journal:  J Drugs Dermatol       Date:  2007-12       Impact factor: 2.114
  7 in total
  4 in total

1.  Comparison of Four Methods for the in vitro Susceptibility Testing of Dermatophytes.

Authors:  Anthi-Marina Markantonatou; Konstantinos Samaras; Evaggelia Zachrou; Timoleon-Achilleas Vyzantiadis
Journal:  Front Microbiol       Date:  2020-07-14       Impact factor: 5.640

2.  N-Chlorotaurine Exhibits Fungicidal Activity against Therapy-Refractory Scedosporium Species and Lomentospora prolificans.

Authors:  Michaela Lackner; Ulrike Binder; Martin Reindl; Beyhan Gönül; Hannes Fankhauser; Christian Mair; Markus Nagl
Journal:  Antimicrob Agents Chemother       Date:  2015-08-03       Impact factor: 5.191

3.  In vitro susceptibility testing for black grain eumycetoma causative agents.

Authors:  Wendy W J van de Sande
Journal:  Trans R Soc Trop Med Hyg       Date:  2021-04-14       Impact factor: 2.184

4.  Molecular Identification and Antifungal Susceptibility Patterns of Clinical Dermatophytes Following CLSI and EUCAST Guidelines.

Authors:  Yubhisha Dabas; Immaculata Xess; Gagandeep Singh; Mragnayani Pandey; Suneeta Meena
Journal:  J Fungi (Basel)       Date:  2017-03-23
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.