Martin Lhuaire1, Romain Tonnelet2, Yohann Renard3, Tullio Piardi4, Daniele Sommacale4, Fabrice Duparc5, Marc Braun2, Marc Labrousse6. 1. Department of Anatomy, Faculté de Médecine, Université de Reims Champagne-Ardenne, Reims, France; Department of General, Digestive and Endocrine Surgery, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Université de Reims Champagne-Ardenne, Reims, France. Electronic address: martin.lhuaire@etudiant.univ-reims.fr. 2. Department of Anatomy, Faculté de Médecine de Nancy, Université de Lorraine, Nancy, France; INSERM U947, Imagerie Adaptative Diagnostique et Interventionnelle (IADI), CHU de Nancy Brabois, Vandoeuvre-lès-Nancy, France. 3. Department of Anatomy, Faculté de Médecine, Université de Reims Champagne-Ardenne, Reims, France; Department of General, Digestive and Endocrine Surgery, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Université de Reims Champagne-Ardenne, Reims, France. 4. Department of General, Digestive and Endocrine Surgery, Hôpital Robert Debré, Centre Hospitalier Universitaire de Reims, Université de Reims Champagne-Ardenne, Reims, France. 5. Department of Anatomy, Faculté de Médecine de Rouen, Université de Rouen, Rouen, France. 6. Department of Anatomy, Faculté de Médecine, Université de Reims Champagne-Ardenne, Reims, France; INSERM U947, Imagerie Adaptative Diagnostique et Interventionnelle (IADI), CHU de Nancy Brabois, Vandoeuvre-lès-Nancy, France.
Abstract
BACKGROUND & AIM: Some aspects of human embryogenesis and organogenesis remain unclear, especially concerning the development of the liver and its vasculature. The purpose of this study was to investigate, from a descriptive standpoint, the evolutionary morphogenesis of the human liver and its vasculature by computerized three-dimensional reconstructions of human embryos. MATERIAL & METHODS: Serial histological sections of four human embryos at successive stages of development belonging to three prestigious French historical collections were digitized and reconstructed in 3D using software commonly used in medical radiology. Manual segmentation of the hepatic anatomical regions of interest was performed section by section. RESULTS: In this study, human liver organogenesis was examined at Carnegie stages 14, 18, 21 and 23. Using a descriptive and an analytical method, we showed that these stages correspond to the implementation of the large hepatic vascular patterns (the portal system, the hepatic artery and the hepatic venous system) and the biliary system. CONCLUSION: To our knowledge, our work is the first descriptive morphological study using 3D computerized reconstructions from serial histological sections of the embryonic development of the human liver between Carnegie stages 14 and 23.
BACKGROUND & AIM: Some aspects of human embryogenesis and organogenesis remain unclear, especially concerning the development of the liver and its vasculature. The purpose of this study was to investigate, from a descriptive standpoint, the evolutionary morphogenesis of the human liver and its vasculature by computerized three-dimensional reconstructions of human embryos. MATERIAL & METHODS: Serial histological sections of four human embryos at successive stages of development belonging to three prestigious French historical collections were digitized and reconstructed in 3D using software commonly used in medical radiology. Manual segmentation of the hepatic anatomical regions of interest was performed section by section. RESULTS: In this study, human liver organogenesis was examined at Carnegie stages 14, 18, 21 and 23. Using a descriptive and an analytical method, we showed that these stages correspond to the implementation of the large hepatic vascular patterns (the portal system, the hepatic artery and the hepatic venous system) and the biliary system. CONCLUSION: To our knowledge, our work is the first descriptive morphological study using 3D computerized reconstructions from serial histological sections of the embryonic development of the human liver between Carnegie stages 14 and 23.
Authors: Martin Lhuaire; Guillaume Wavreille; Mikael Hivelin; Aurélien Aumar; Vincent Hunsinger; Mohamed Derder; Alexandre G Lellouch; Peter Abrahams; Laurent Lantieri; Christian Fontaine Journal: JPRAS Open Date: 2022-05-14
Authors: Jill P J M Hikspoors; Mathijs M J P Peeters; Nutmethee Kruepunga; Hayelom K Mekonen; Greet M C Mommen; S Eleonore Köhler; Wouter H Lamers Journal: Sci Rep Date: 2017-12-07 Impact factor: 4.379