Mehmet Arasli1, Yasemin Ozsurekci2, Nazif Elaldi3, Alexander J McAuley4, Eda Karadag Oncel2, Ishak Ozel Tekin5, Mustafa Gokhan Gozel3, Ali Kaya6, Fusun Dilara Icagasioglu6, Dilek Yagci Caglayik7, Gulay Korukluoglu7, Furuzan Kokturk8, Mehmet Bakir3, Dennis A Bente4, Mehmet Ceyhan2. 1. Department of Immunology, School of Medicine, Bulent Ecevit University, Zonguldak, Turkey. Electronic address: araslimehmet@yahoo.com. 2. Department of Paediatric Infectious Diseases, School of Medicine, Hacettepe University, Ankara, Turkey. 3. Department of Infectious Diseases and Clinical Microbiology, School of Medicine, Cumhuriyet University, Sivas, Turkey. 4. Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA; Department of Microbiology & Immunology, University of Texas Medical Branch,Galveston, TX, USA. 5. Department of Immunology, School of Medicine, Bulent Ecevit University, Zonguldak, Turkey. 6. Department of Paediatrics, School of Medicine, Cumhuriyet University, Sivas, Turkey. 7. Refik Saydam National Public Health Agency, Virology Reference and Research Laboratory, Ankara, Turkey. 8. Department of Biostatistics, School of Medicine, Bulent Ecevit University, Zonguldak, Turkey.
Abstract
BACKGROUND: Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral zoonosis. Clinical reports indicate the severity of CCHF is milder in children than adults. The chemokines are important chemo-attractant mediators of the host immune system. OBJECTIVES: The main aim of the study was to identify whether or not there were any differences in chemokine levels between the pediatric and adult patients and control groups, and whether there was any correlation with disease severity. STUDY DESIGN: The serum levels of select chemokines including chemokine (C-C) ligand 2 (CCL2), CCL3, CCL4, chemokine (C-X-C) ligand 8 (CXCL8), CXCL9, and granulocyte-colony stimulating factor (G-CSF) in 29 adult and 32 pediatric CCHF patients and in 35 healthy children and 40 healthy adult control groups were studied by flow cytometric bead immunoassay method. RESULTS: Great variability was detected in the serum levels of the chemokines for both the adult and pediatric patients and controls. With the exception of G-CSF, the median serum levels of CCL2, CCL3, CCL4, CXCL8, and CXCL9 were found to be significantly higher in the adult patients compared to adult controls (2364.7 vs. 761 pg/ml; 714.1 vs. 75.2 pg/ml; 88.6 vs. 25.5 pg/ml; 217.9 vs. 18.3 pg/ml; 875 vs. 352.2 pg/ml, respectively, p < 0.0001 for all comparisons). Among the chemokines the median CCL4 and G-CSF levels were significantly higher in the pediatric patients compared to pediatric controls (40.3 vs. 7.1 pg/ml, p < 0.0001; 0.1 vs. 0.1 pg/ml, p = 0.049, respectively). CONCLUSION: The results of this study showed prominent chemokine raising in adult CCHF patients compared to children CCHF patients.
BACKGROUND:Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral zoonosis. Clinical reports indicate the severity of CCHF is milder in children than adults. The chemokines are important chemo-attractant mediators of the host immune system. OBJECTIVES: The main aim of the study was to identify whether or not there were any differences in chemokine levels between the pediatric and adult patients and control groups, and whether there was any correlation with disease severity. STUDY DESIGN: The serum levels of select chemokines including chemokine (C-C) ligand 2 (CCL2), CCL3, CCL4, chemokine (C-X-C) ligand 8 (CXCL8), CXCL9, and granulocyte-colony stimulating factor (G-CSF) in 29 adult and 32 pediatric CCHFpatients and in 35 healthy children and 40 healthy adult control groups were studied by flow cytometric bead immunoassay method. RESULTS: Great variability was detected in the serum levels of the chemokines for both the adult and pediatric patients and controls. With the exception of G-CSF, the median serum levels of CCL2, CCL3, CCL4, CXCL8, and CXCL9 were found to be significantly higher in the adult patients compared to adult controls (2364.7 vs. 761 pg/ml; 714.1 vs. 75.2 pg/ml; 88.6 vs. 25.5 pg/ml; 217.9 vs. 18.3 pg/ml; 875 vs. 352.2 pg/ml, respectively, p < 0.0001 for all comparisons). Among the chemokines the median CCL4 and G-CSF levels were significantly higher in the pediatric patients compared to pediatric controls (40.3 vs. 7.1 pg/ml, p < 0.0001; 0.1 vs. 0.1 pg/ml, p = 0.049, respectively). CONCLUSION: The results of this study showed prominent chemokine raising in adult CCHF patients compared to children CCHF patients.
Authors: Robert A Kozak; Russell S Fraser; Mia J Biondi; Anna Majer; Sarah J Medina; Bryan D Griffin; Darwyn Kobasa; Patrick J Stapleton; Chantel Urfano; Giorgi Babuadze; Kym Antonation; Lisa Fernando; Stephanie Booth; Brandon N Lillie; Gary P Kobinger Journal: PLoS Negl Trop Dis Date: 2020-04-06
Authors: Joel Henrique Ellwanger; Bruna Kulmann-Leal; Valéria de Lima Kaminski; Andressa Gonçalves Rodrigues; Marcelo Alves de Souza Bragatte; José Artur Bogo Chies Journal: Virus Res Date: 2020-05-30 Impact factor: 3.303