Ke Li1, Chi Zhang1, Rong Zhao1, Ying Xue1, Jian Yang1, Junping Peng2, Qi Jin3. 1. MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. 2. MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. Electronic address: pengjp@gmail.com. 3. MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. Electronic address: zdsys@vip.sina.com.
Abstract
BACKGROUND: Over the past 7 years, eleven novel human polyomaviruses (HPyVs) have been identified. The frequent discovery of human polyomaviruses (HPyVs) in the gastrointestinal tract and stool samples suggests a potential involvement in gastroenteritis. OBJECTIVE: In this study we want to explore the prevalence of STL polyomavirus (STLPyV) in China and delineate the clinical role played by STLPyV. STUDY DESIGN: Stool samples from 508 hospitalized children with diarrhea and 271 healthy children were screened to detect STLPyV. Human polyomavirus 12(HPyV12), New Jersey polyomavirus (NJPyV-2013) and six common enteric viruses (including rotaviruses, adenovirus, norovirus GI and GII, astrovirus and sapovirus) were also screened in this study. RESULTS: 348 of the 508 (68.5%) specimens from the hospitalized children with diarrhea contained at least 1 common enteric virus. STLPyV was identified in 11 specimens in the case group (2.2%), among which 4 specimens were negative for those common enteric viruses. STLPyV was not more prevalent among the case group than the control group (2.2% versus 3.0%; p = 0.50, χ(2) test). In case group, when common enteric viruses' positive and negative groups were compared, the difference in detection rate of STLPyV was not statistically significant (2.5% versus 2.0%; p = 0.98, χ(2) test). Two whole genome sequences of STLPyV were obtained. CONCLUSIONS: We are the first to report the prevalence of STLPyV in Chinese children and obtained whole genome sequences of STLPyV strains isolated in China. Our results of phylogenetic analysis support the hypothesis that STLPyV is geographically widespread.
BACKGROUND: Over the past 7 years, eleven novel humanpolyomaviruses (HPyVs) have been identified. The frequent discovery of humanpolyomaviruses (HPyVs) in the gastrointestinal tract and stool samples suggests a potential involvement in gastroenteritis. OBJECTIVE: In this study we want to explore the prevalence of STL polyomavirus (STLPyV) in China and delineate the clinical role played by STLPyV. STUDY DESIGN: Stool samples from 508 hospitalized children with diarrhea and 271 healthy children were screened to detect STLPyV. Human polyomavirus 12(HPyV12), New Jersey polyomavirus (NJPyV-2013) and six common enteric viruses (including rotaviruses, adenovirus, norovirus GI and GII, astrovirus and sapovirus) were also screened in this study. RESULTS: 348 of the 508 (68.5%) specimens from the hospitalized children with diarrhea contained at least 1 common enteric virus. STLPyV was identified in 11 specimens in the case group (2.2%), among which 4 specimens were negative for those common enteric viruses. STLPyV was not more prevalent among the case group than the control group (2.2% versus 3.0%; p = 0.50, χ(2) test). In case group, when common enteric viruses' positive and negative groups were compared, the difference in detection rate of STLPyV was not statistically significant (2.5% versus 2.0%; p = 0.98, χ(2) test). Two whole genome sequences of STLPyV were obtained. CONCLUSIONS: We are the first to report the prevalence of STLPyV in Chinese children and obtained whole genome sequences of STLPyV strains isolated in China. Our results of phylogenetic analysis support the hypothesis that STLPyV is geographically widespread.