AIM: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. MATERIALS & METHODS: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. RESULTS: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. CONCLUSION: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.
AIM: To develop emerging diagnostic technique for bovinetuberculosis and to identify its potential risk factors. MATERIALS & METHODS: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. RESULTS: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. CONCLUSION: Results of the study may add value to bovinetuberculosis eradication campaigns to achieve the One Health initiative.
Authors: Sk Shaheenur Islam; Tanzida Begum Rumi; S M Lutful Kabir; A K M Anisur Rahman; Md Mahmudul Hasan Faisal; Robiul Islam; Adri G M van der Zanden; Michael P Ward; Allen G Ross; Zeaur Rahim Journal: PLoS Negl Trop Dis Date: 2021-04-30
Authors: Sukanta Chowdhury; Mohammad A Aleem; Md Shafiqul I Khan; Mohammad Enayet Hossain; Sumon Ghosh; Mohammed Z Rahman Journal: Vet Med Sci Date: 2021-03-02
Authors: K Renuga Devi; L J Lee; Lee Tze Yan; Amin-Nordin Syafinaz; I Rosnah; V K Chin Journal: Int Arch Occup Environ Health Date: 2021-03-16 Impact factor: 3.015