Literature DB >> 2586500

[Determination of the substrate specificity of Bpu101 restrictase with an unusual recognition segment].

S Kh Degtiarev, P A Zhilkin, G G Prikhod'ko, N I Rechkunova, V E Repin.   

Abstract

A new enzyme Bpu10I was isolated from Bacillus pumilus. This enzyme is not an isoschizomer of any known restriction endonucleases. The search of possible recognition sequences was carried out in sequences ABCNiDEF (i = 0.6) on substrate DNA lambda CI857, T7, pBR322. The recognition sequence and cleavage sites of restriction endonuclease Bpu10I have been determined as CCTNAGC. GGANTCG

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Year:  1989        PMID： 2586500

Source DB:  PubMed          Journal:  Mol Biol (Mosk)        ISSN： 0026-8984


  5 in total

1.  BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5'-CACGTC(-3/-3)-3'.

Authors:  S K Degtyarev; O A Belichenko; N A Lebedeva; V S Dedkov; M A Abdurashitov
Journal:  Nucleic Acids Res       Date:  2000-06-01       Impact factor: 16.971

2.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

3.  Cloning and analysis of the four genes coding for Bpu10I restriction-modification enzymes.

Authors:  K Stankevicius; A Lubys; A Timinskas; D Vaitkevicius; A Janulaitis
Journal:  Nucleic Acids Res       Date:  1998-02-15       Impact factor: 16.971

4.  II-Q restriction endonucleases--new class of type II enzymes.

Authors:  S K Degtyarev; N I Rechkunova; A A Kolyhalov; V S Dedkov; P A Zhilkin
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

5.  Mapping the nicking efficiencies of nickase R.BbvCI for side-specific LNA-substituted substrates using rolling circle amplification.

Authors:  Hua Wei; Guojie Zhao; Tianyu Hu; Suming Tang; Jiquan Jiang; Bo Hu; Yifu Guan
Journal:  Sci Rep       Date:  2016-09-01       Impact factor: 4.379
  5 in total

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