Helmut Bühler1, Gerfried L Strohm2, Pascaline Nguemgo-Kouam3, Hans Lamm4, Khashayar Fakhrian2, Irenäus A Adamietz2. 1. Institute for Molecular Oncology, Radio-Biology and Experimental Radiotherapy, University Medical Centre Marienhospital, Ruhr-University Bochum, Herne, Germany helmut.buehler@rub.de. 2. Department of Radiotherapy and Radio-Oncology, University Medical Centre Marienhospital, Ruhr-University Bochum, Herne, Germany. 3. Institute for Molecular Oncology, Radio-Biology and Experimental Radiotherapy, University Medical Centre Marienhospital, Ruhr-University Bochum, Herne, Germany. 4. Druckkammerzentrum Hannover, Hannover, Germany.
Abstract
BACKGROUND: Hyperbaric oxygen (HBO) seems to intensify the effect of ionising radiation. We investigated whether HBO combined with irradiation decreases the capability of U251 glioblastoma cells for relapse and metastasis. MATERIALS AND METHODS: Cells were treated with O2 at 1.3 bar and then irradiated with 2 Gy photons. Clonogenic survival was tested with colony formation. Motility is an important feature of metastasis and was measured with time-lapse videography. RESULTS: The clonogenic survival diminished by 22% through HBO, by 49% through irradiation, and by 70% through the combination of both. The accumulated distance travelled by cells fell by 3% with HBO, rose by 17% with irradiation, but was reduced by 11% with their combination. The respective values for the Euclidean distance travelled were +8%, +47% and -14%. Compared to normoxic irradiation, additional HBO lowered travel by 41%. CONCLUSION: HBO strengthens the effect of irradiation on clonogenic survival and reverses radiation-induced increase in the mobility of cells. Copyright
BACKGROUND: Hyperbaric oxygen (HBO) seems to intensify the effect of ionising radiation. We investigated whether HBO combined with irradiation decreases the capability of U251 glioblastoma cells for relapse and metastasis. MATERIALS AND METHODS: Cells were treated with O2 at 1.3 bar and then irradiated with 2 Gy photons. Clonogenic survival was tested with colony formation. Motility is an important feature of metastasis and was measured with time-lapse videography. RESULTS: The clonogenic survival diminished by 22% through HBO, by 49% through irradiation, and by 70% through the combination of both. The accumulated distance travelled by cells fell by 3% with HBO, rose by 17% with irradiation, but was reduced by 11% with their combination. The respective values for the Euclidean distance travelled were +8%, +47% and -14%. Compared to normoxic irradiation, additional HBO lowered travel by 41%. CONCLUSION: HBO strengthens the effect of irradiation on clonogenic survival and reverses radiation-induced increase in the mobility of cells. Copyright