Potential risk of isoflavones: toxicological study of daidzein supplementation in piglets.

As a main component of soy isoflavones, daidzein is rich in soy-derived products, which are widely used as feed ingredients in farm animals. However, little research has been conducted on the side effects of dietary daidzein, especially in young animals. In this study, the safety of daidzein was evaluated. Results show that ingesting 400 mg/kg of dietary daidzein for 70 days is associated with a lower average daily weight gain (kilogram) (0.47 ± 0.03 vs 0.54 ± 0.04, P < 0.05) and a higher splenic damage index (1.00 ± 1.10 vs 0.00 ± 0.00, P < 0.05) in young pigs compared with control. Female pigs receiving 200 and 400 mg/kg daidzein showed reduced serum testosterone levels (ng/L) on days 35 and 70 compared with the control group (day 35, 246 ± 74 and 224 ± 20 vs 362 ± 48, P < 0.05; day 70, 252 ± 38 and 219 ± 77 vs 374 ± 38, P < 0.05). Daidzein residue (μg/kg) in pig livers increased (243 ± 80 vs 142 ± 47, P < 0.05, day 70). These results suggest that dietary supplements of 400 mg/kg of daidzein negatively affect the weight gain and splenic morphology of pigs.