| Literature DB >> 25860243 |
Francesco Maura1, Giovanna Cutrona2, Laura Mosca1, Serena Matis3, Marta Lionetti1, Sonia Fabris1, Luca Agnelli1, Monica Colombo3, Carlotta Massucco3, Manuela Ferracin4, Barbara Zagatti4, Daniele Reverberi5, Massimo Gentile6, Anna Grazia Recchia6, Sabrina Bossio6, Davide Rossi7, Gianluca Gaidano7, Stefano Molica8, Agostino Cortelezzi1, Francesco Di Raimondo9, Massimo Negrini4, Pierfrancesco Tassone10, Fortunato Morabito6, Manlio Ferrarini3, Antonino Neri1.
Abstract
In this study we investigated specific biological and clinical features associated with chronic lymphocytic leukemia (CLL) patients carrying stereotyped BCR subset #4 (IGHV4-34) among a prospective cohort of 462 CLL/MBL patients in early stage (Binet A). All subset #4 patients (n = 16) were characterized by the IGHV mutated gene configuration, and absence of unfavorable cytogenetic lesions, NOTCH1 or SF3B1 mutations. Gene and miRNA expression profiling evidenced that the leukemic cells of subset #4 cases showed significant downregulation of WDFY4, MF2A and upregulation of PDGFA, FGFR1 and TFEC gene transcripts, as well as the upregulation of miR-497 and miR-29c. The transfection of miR-497 mimic in primary leukemic CLL cells induced a downregulation of BCL2, a known validated target of this miRNA. Our data identify biological characteristics associated with subset #4 patients, providing further evidence for the putative role of BCR in shaping the features of the tumor cells in CLL.Entities:
Keywords: B-cell receptor; BCL2; chronic lymphocytic leukemia; gene expression profiling; microRNA; stereotyped VH CDR
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Year: 2015 PMID： 25860243 DOI： 10.3109/10428194.2015.1028051
Source DB: PubMed Journal: Leuk Lymphoma ISSN： 1026-8022