Mesut Sipahi1, Kürşad Zengin2, Serhat Tanik3, Ergin Arslan1, Anıl Çubukçu4. 1. Department of General Surgery, Faculty of Medicine, Bozok University, Yozgat, Turkey. 2. Department of Urology, Faculty of Medicine, Bozok University, Yozgat, Turkey; email: kursadzengin@gmail.com. 3. Department of Urology, Faculty of Medicine, Bozok University, Yozgat, Turkey. 4. Department of General Surgery, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey.
Abstract
INTRODUCTION: Many body systems, especially the immune system, are affected by circadian rhythm disorders (CRDs). This study investigated the effects of shift lag on wound healing and bowel anastomosis in rats. MATERIALS AND METHODS: Forty-five rats were randomly divided into 3 groups: placebo, control, and experimental. Circadian rhythm was disturbed with altered light/dark cycles. Colon anastomosis was performed in rats with and without disturbed circadian rhythms. Serum melatonin levels and bowel anastomosis bursting pressures were measured. Tissue samples were used to measure tissue hydroxyproline levels and for histological assessment. RESULTS: In the groups with an altered circadian rhythm, the bowel bursting pressures, tissue hydroxyproline levels, and fibrosis were greater than the control group. CONCLUSION: This study found that a CRD, simulated by altered light/dark cycles, positively affected the anastomotic bursting pressure. This suggests that cytokines such as interleukin (IL)-1, IL-6, transforming growth factor α, and stress hormones affect fibroblasts and thereby increase collagen synthesis in the proliferative phase. More studies are necessary to understand the effects of CRDs. .
INTRODUCTION: Many body systems, especially the immune system, are affected by circadian rhythm disorders (CRDs). This study investigated the effects of shift lag on wound healing and bowel anastomosis in rats. MATERIALS AND METHODS: Forty-five rats were randomly divided into 3 groups: placebo, control, and experimental. Circadian rhythm was disturbed with altered light/dark cycles. Colon anastomosis was performed in rats with and without disturbed circadian rhythms. Serum melatonin levels and bowel anastomosis bursting pressures were measured. Tissue samples were used to measure tissue hydroxyproline levels and for histological assessment. RESULTS: In the groups with an altered circadian rhythm, the bowel bursting pressures, tissue hydroxyproline levels, and fibrosis were greater than the control group. CONCLUSION: This study found that a CRD, simulated by altered light/dark cycles, positively affected the anastomotic bursting pressure. This suggests that cytokines such as interleukin (IL)-1, IL-6, transforming growth factor α, and stress hormones affect fibroblasts and thereby increase collagen synthesis in the proliferative phase. More studies are necessary to understand the effects of CRDs. .
Authors: Santiago Elías Charif; Pablo Ignacio Felipe Inserra; Alejandro Raúl Schmidt; Santiago Andrés Cortasa; Sofía Proietto; María Clara Corso; Julia Halperin; Noelia Paula Di Giorgio; Victoria Lux-Lantos; Alfredo Daniel Vitullo; Verónica Berta Dorfman Journal: J Comp Physiol B Date: 2021-08-30 Impact factor: 2.200