Literature DB >> 25854484

Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing.

Alexandra Y C Kruse1, Lasse Kvich2, Steffen Eickhardt2, Lars H Omland3, Thomas Bjarnsholt4, Claus Moser5.   

Abstract

The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID: 25854484      PMCID: PMC4432055          DOI: 10.1128/JCM.03404-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  10 in total

Review 1.  Toxoplasmosis: diagnosis, treatment, and prevention in congenitally exposed infants.

Authors:  Alyson Kaye
Journal:  J Pediatr Health Care       Date:  2010-06-12       Impact factor: 1.812

Review 2.  Toxoplasmosis in the adult.

Authors:  J S Remington
Journal:  Bull N Y Acad Med       Date:  1974-02

Review 3.  AIDS commentary. Toxoplasmic encephalitis.

Authors:  B J Luft; J S Remington
Journal:  J Infect Dis       Date:  1988-01       Impact factor: 5.226

Review 4.  Polymerase chain reaction approaches for the detection of Neospora caninum and Toxoplasma gondii.

Authors:  J T Ellis
Journal:  Int J Parasitol       Date:  1998-07       Impact factor: 3.981

5.  Dietzia papillomatosis bacteremia.

Authors:  Paul Rammer; Henrik Calum; Claus Moser; Maria K Björnsdóttir; Heidi Smedegaard; Niels Høiby; Thomas Bjarnsholt
Journal:  J Clin Microbiol       Date:  2013-03-27       Impact factor: 5.948

6.  Toxoplasmosis of the central nervous system in the acquired immunodeficiency syndrome.

Authors:  S B Porter; M A Sande
Journal:  N Engl J Med       Date:  1992-12-03       Impact factor: 91.245

Review 7.  Toxoplasmic encephalitis in AIDS.

Authors:  B J Luft; J S Remington
Journal:  Clin Infect Dis       Date:  1992-08       Impact factor: 9.079

Review 8.  Toxoplasmosis snapshots: global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis.

Authors:  Georgios Pappas; Nikos Roussos; Matthew E Falagas
Journal:  Int J Parasitol       Date:  2009-05-09       Impact factor: 3.981

Review 9.  Toxoplasma gondii: transmission, diagnosis and prevention.

Authors:  D Hill; J P Dubey
Journal:  Clin Microbiol Infect       Date:  2002-10       Impact factor: 8.067

10.  Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes.

Authors:  Udo Reischl; Stéphane Bretagne; Dominique Krüger; Pauline Ernault; Jean-Marc Costa
Journal:  BMC Infect Dis       Date:  2003-05-02       Impact factor: 3.090

  10 in total
  2 in total

1.  Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples.

Authors:  Camilla Stavnsbjerg; Niels Frimodt-Møller; Claus Moser; Thomas Bjarnsholt
Journal:  BMC Infect Dis       Date:  2017-03-27       Impact factor: 3.090

2.  Application of stereotactic biopsy for diagnosing intracranial lesions in patients with AIDS in China: Report of 7 cases.

Authors:  Ji-Bo Zhang; Kai Fu; Rui Gong; Xue-Meng Liu; Li-Dao Chen; Yong-Xi Zhang; Gui-Fang Yang; Jie Zhang
Journal:  Medicine (Baltimore)       Date:  2016-12       Impact factor: 1.817

  2 in total

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