Jian Tao Huang1, Guo Dong Wang2, Da Lin Wang3, Yuan Liu4, Xu Yun Zhang5, Yun Fu Zhao6. 1. Department of Stomatology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Rd, Shanghai 200003, PR China. Electronic address: 496349641@qq.com. 2. Department of Stomatology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Rd, Shanghai 200003, PR China. Electronic address: louis_w@126.com. 3. Department of Stomatology Changhai Hospital, Second Military Medical University, 168 Changhai Rd, Shanghai 200438, PR China. Electronic address: Wang_Dento@163.com. 4. Department of Stomatology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Rd, Shanghai 200003, PR China. Electronic address: liuyuan_6@hotmail.com. 5. Department of Stomatology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Rd, Shanghai 200003, PR China; Pudong New Area Eye and Dental Disease Prevention and Treatment Center, 222 Wenhua Rd, Huinan Town District, Pudong New Area, Shanghai 201399, PR China. Electronic address: hw55666123@163.com. 6. Department of Stomatology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Rd, Shanghai 200003, PR China. Electronic address: zhaoyf1818@126.com.
Abstract
BACKGROUND: Previous studies of vibrissal movements employ either optoelectronic recording techniques in the head fixed rodent, or videographic recordings in freely moving animals. However, both approaches have shortcomings for quantitatively tracking the process of vibrissal motor recovery. NEW METHOD: A critical feature of our videographic method is to measure tagged vibrissae movements while leaving all others intact in body restrained rats without head fixation. Thirty two adult rats underwent facial nerve manipulation and testing. All animals underwent baseline preoperative whisking testing. In the experimental groups, the right facial nerve was either crushed, or transected and sutured. In the control groups, the left facial nerve underwent either sham surgery, or transection denervation. Whisking function was measured for the ensuing 2 to 12 weeks. Data were analyzed for whisking recovery. RESULTS: Baseline preoperative whisking testing showed that majority of free whisking on the both sides is synchronous and symmetric, which allows us to compare vibrissal motor data between intact and manipulated side after facial nerve injury. As expected, the recovery of whisking function following crush is better and earlier than that with transection and suture. COMPARISON WITH EXISTING METHOD(S): To our knowledge, this novel videographic method is a significant simplification over currently employed optoelectronic recording techniques and videographic methods. CONCLUSIONS: Our novel videographic method may be a powerful tool to investigate motor recovery from facial nerve manipulation in the rat model.
BACKGROUND: Previous studies of vibrissal movements employ either optoelectronic recording techniques in the head fixed rodent, or videographic recordings in freely moving animals. However, both approaches have shortcomings for quantitatively tracking the process of vibrissal motor recovery. NEW METHOD: A critical feature of our videographic method is to measure tagged vibrissae movements while leaving all others intact in body restrained rats without head fixation. Thirty two adult rats underwent facial nerve manipulation and testing. All animals underwent baseline preoperative whisking testing. In the experimental groups, the right facial nerve was either crushed, or transected and sutured. In the control groups, the left facial nerve underwent either sham surgery, or transection denervation. Whisking function was measured for the ensuing 2 to 12 weeks. Data were analyzed for whisking recovery. RESULTS: Baseline preoperative whisking testing showed that majority of free whisking on the both sides is synchronous and symmetric, which allows us to compare vibrissal motor data between intact and manipulated side after facial nerve injury. As expected, the recovery of whisking function following crush is better and earlier than that with transection and suture. COMPARISON WITH EXISTING METHOD(S): To our knowledge, this novel videographic method is a significant simplification over currently employed optoelectronic recording techniques and videographic methods. CONCLUSIONS: Our novel videographic method may be a powerful tool to investigate motor recovery from facial nerve manipulation in the rat model.
Authors: Jonathan Krynitsky; Alex A Legaria; Julia J Pai; Marcial Garmendia-Cedillos; Ghadi Salem; Tom Pohida; Alexxai V Kravitz Journal: eNeuro Date: 2020-05-12