Özlem Beyazyildiz1, Mehmet Hakan Tirhiş2, Emine Rümeysa Hekimoğlu3, Emrullah Beyazyildiz1, Figen Kaymaz3, Pelin Yilmazbaş2, Faruk Öztürk4. 1. a Department of Ophthalmology , Samsun Research and Training Hospital , Samsun , Turkey . 2. b Department of Ophthalmology , Ulucanlar Eye Research and Training Hospital , Ankara , Turkey . 3. c Department of Histology , Hacettepe University , Ankara , Turkey and. 4. d Department of Ophthalmology , Atatürk Research and Training Hospital , Ankara , Turkey.
Abstract
PURPOSE: To investigate histopathological changes of internal limiting membrane (ILM) in patients with epiretinal membrane (ERM) Materials and Methods:Forty-two eyes of 42 patients who were diagnosed as ERM and enrolled for vitreoretinal surgery were included in this study. Brilliant Blue G (BBG) was used to stain the ILM in all patients. ILM was peeled in all subjects and analyzed by light microscopy (methylene blue-Azur II × 40). ILM samples were then fixed in 2.5% glutaraldehyde solution and examined in JEOL-JEM 1400 and 2100F electron microscope and photographed by CCD camera (Gatan Inc., Pleasanton, CA). RESULTS: Remained ERM fragments were observed on 80% of ILM's. Vacuolization of ILM was observed in a patient with diabetic ERM. There were cells and cellular fragments observed mostly at retinal side of ILM which was likely to be a fragment of Muller cells of retina. CONCLUSIONS: Most of the ILM's had residual ERM tissue and contained cells and cellular fragments at retinal side of ILM's. ILM peeling might have a role in decreasing ERM recurrence by removal of residual ERM tissues.
RCT Entities:
PURPOSE: To investigate histopathological changes of internal limiting membrane (ILM) in patients with epiretinal membrane (ERM) Materials and Methods: Forty-two eyes of 42 patients who were diagnosed as ERM and enrolled for vitreoretinal surgery were included in this study. Brilliant Blue G (BBG) was used to stain the ILM in all patients. ILM was peeled in all subjects and analyzed by light microscopy (methylene blue-Azur II × 40). ILM samples were then fixed in 2.5% glutaraldehyde solution and examined in JEOL-JEM 1400 and 2100F electron microscope and photographed by CCD camera (Gatan Inc., Pleasanton, CA). RESULTS: Remained ERM fragments were observed on 80% of ILM's. Vacuolization of ILM was observed in a patient with diabetic ERM. There were cells and cellular fragments observed mostly at retinal side of ILM which was likely to be a fragment of Muller cells of retina. CONCLUSIONS: Most of the ILM's had residual ERM tissue and contained cells and cellular fragments at retinal side of ILM's. ILM peeling might have a role in decreasing ERM recurrence by removal of residual ERM tissues.
Entities:
Keywords:
Electron microscopy; epiretinal membrane; internal limiting membrane; retina; vitreoretinal surgery