Qiu-xian Peng1,2, Hong-bing Cai1, Jiang-li Peng3, Kin Lam Yung2, Jue Shi2, Zhi-xian Mo4. 1. School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China. 2. Faculty of Science, Hong Kong Baptist University, Hong Kong SAR, China. 3. College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China. 4. School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China. cherrymo@fimmu.com.
Abstract
OBJECTIVE: To observe the effects of water extract of Zuojin Pill ([characters: see text], ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. METHODS: Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9. RESULTS: ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation. CONCLUSIONS: ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.
OBJECTIVE: To observe the effects of water extract of Zuojin Pill ([characters: see text], ZJP) on inhibiting the growth of humangastric cancer cell line SGC-7901 and its potential mechanism. METHODS: Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9. RESULTS:ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation. CONCLUSIONS:ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.
Entities:
Keywords:
Chinese medicine; Zuojin Pill; apoptosis; mitochondria-dependent pathway; reactive oxygen species
Authors: Manoj K Pandey; Bokyung Sung; Ajaikumar B Kunnumakkara; Gautam Sethi; Madan M Chaturvedi; Bharat B Aggarwal Journal: Cancer Res Date: 2008-07-01 Impact factor: 12.701