| Literature DB >> 25847692 |
Yuanpeng Zhang1, Jiao Jing1, Xinfeng Li1, Jingman Wang1, Xiuli Feng1, Ruibing Cao2, Puyan Chen3.
Abstract
To elucidate the role of microRNAs (miRNA) in the regulation of gene expression in Japanese encephalitis virus (JEV) infected swine testis (ST) cells, we analyzed miRNA and mRNA expression profiles of JEV infected ST cells by high-throughput sequencing technology as compared to uninfected controls. The results showed that 104 known miRNAs and 9 new miRNA candidates were differentially expressed in ST cells after JEV infection. We identified 396 differentially expressed mRNAs. Bioinformatics analysis identified 435 known miRNA-mRNA interaction pairs and 94 novel miRNA-mRNA interaction pairs involving miRNAs inversely correlated with the expression of their predicted target mRNAs. The known miRNAs inversely correlated with their target genes were involved in the biological processes of immunity, cytokine production, inflammation, and apoptosis. Selected miRNA-mRNA interactions were validated by luciferase reporter assay. Overall, our findings indicate that miRNAs may play critical roles in the pathogenesis of JEV infection.Entities:
Keywords: Integration analysis; Japanese encephalitis virus; MicroRNA; mRNA
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Year: 2015 PMID： 25847692 DOI： 10.1016/j.meegid.2015.03.037
Source DB: PubMed Journal: Infect Genet Evol ISSN： 1567-1348 Impact factor: 3.342