Stella Maris de Freitas Lima1, Maurício Gonçalves da Costa Sousa2, Mirna de Souza Freire3, Jeeser Alves de Almeida4, Ana Paula de Castro Cantuária2, Thaís Angélica Machado E Silva1, Camila Guimarães de Freitas5, Simoni Campos Dias6, Octávio Luiz Franco7, Taia Maria Berto Rezende8. 1. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Curso de Odontologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil. 2. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil. 3. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Programa de Pós-Graduação da Rede Centro-Oeste, Brasília, Distrito Federal, Brazil. 4. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Curso de Educação Física, Universidade Federal de Mato Grosso do Sul - UFMS, Campo Grande, Mato Grosso do Sul, Brazil. 5. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Instituto Federal de Brasília, Brasília, Distrito Federal, Brazil. 6. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil. 7. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; S-Inova, Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil. 8. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Curso de Odontologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil. Electronic address: taiambr@gmail.com.
Abstract
INTRODUCTION: Persistent microorganisms such as Candida albicans and Enterococcus faecalis might be directly related to endodontic treatment failure. The host response to these microorganisms impairs the reestablishment of intraradicular and periradicular health. METHODS: The present investigation evaluated the expression of inflammatory mediators produced by RAW 264.7 cells in the presence of heat-killed antigens (HK) C. albicans and E. faecalis. Cultures of RAW cells were stimulated with both antigens in the presence or absence of recombinant interferon (rIFN)-γ. Parameters of cell viability, production of nitric oxide (NO), as well as the synthesis of interleukin (IL)-1α, IL-6, IL-10, IL-12, monocyte chemotactic protein-1, and tumor necrosis factor (TNF)-α were analyzed. RESULTS: Results demonstrated that cell viability was especially reduced in antigens and rIFN-γ-stimulated groups. Groups stimulated with HK C. albicans upregulated IL-10 production. Otherwise, the addition of rIFN-γ to HK C. albicans upregulated TNF-α and NO production. Groups stimulated with HK E. faecalis upregulated TNF-α production. HK E. faecalis and rIFN-γ upregulated TNF-α and NO synthesis. The production of other cytokines remained unchanged by all stimuli. CONCLUSIONS: Knowledge regarding the host mechanism of response to microorganisms that perpetuate endodontic infection and the periradicular lesions can contribute to optimization of endodontic therapy. The mentioned inflammatory mediators and virulence factors involved in endodontic failure might guide lesion progression and also be targets in the development of disinfectant and immunomodulatory agents.
INTRODUCTION: Persistent microorganisms such as Candida albicans and Enterococcus faecalis might be directly related to endodontic treatment failure. The host response to these microorganisms impairs the reestablishment of intraradicular and periradicular health. METHODS: The present investigation evaluated the expression of inflammatory mediators produced by RAW 264.7 cells in the presence of heat-killed antigens (HK) C. albicans and E. faecalis. Cultures of RAW cells were stimulated with both antigens in the presence or absence of recombinant interferon (rIFN)-γ. Parameters of cell viability, production of nitric oxide (NO), as well as the synthesis of interleukin (IL)-1α, IL-6, IL-10, IL-12, monocyte chemotactic protein-1, and tumor necrosis factor (TNF)-α were analyzed. RESULTS: Results demonstrated that cell viability was especially reduced in antigens and rIFN-γ-stimulated groups. Groups stimulated with HK C. albicans upregulated IL-10 production. Otherwise, the addition of rIFN-γ to HK C. albicans upregulated TNF-α and NO production. Groups stimulated with HK E. faecalis upregulated TNF-α production. HK E. faecalis and rIFN-γ upregulated TNF-α and NO synthesis. The production of other cytokines remained unchanged by all stimuli. CONCLUSIONS: Knowledge regarding the host mechanism of response to microorganisms that perpetuate endodontic infection and the periradicular lesions can contribute to optimization of endodontic therapy. The mentioned inflammatory mediators and virulence factors involved in endodontic failure might guide lesion progression and also be targets in the development of disinfectant and immunomodulatory agents.
Authors: Camila G Freitas; Stella M F Lima; Mirna S Freire; Ana Paula C Cantuária; Nelson G O Júnior; Tatiane S Santos; Jéssica S Folha; Suzana M Ribeiro; Simoni C Dias; Taia M B Rezende; Patrícia Albuquerque; André M Nicola; César de la Fuente-Núñez; Robert E W Hancock; Octávio L Franco; Maria Sueli S Felipe Journal: Antimicrob Agents Chemother Date: 2017-07-25 Impact factor: 5.191
Authors: Nathalia F Camarço; Ivo V Sousa Neto; Dahan C Nascimento; Jeeser A Almeida; Denis C L Vieira; Thiago S Rosa; Guilherme B Pereira; Jonato Prestes Journal: J Clin Transl Res Date: 2016-06-19
Authors: Om Alkhir Alshanta; Khawlah Albashaireh; Emily McKloud; Christopher Delaney; Ryan Kean; William McLean; Gordon Ramage Journal: Biofilm Date: 2022-03-14
Authors: Mauricio Gonçalves da Costa Sousa; Gabriela Conceição de Almeida; Danilo César Martins Mota; Rosiane Andrade da Costa; Simoni Campos Dias; Samuel Nunes Limberger; Frank Ko; Li Ting Lin; Evan F Haney; Hashem Etayash; Beverlie Baquir; Michael J Trimble; Ya Shen; Zheng Su; Markus Haapasalo; Daniel Pletzer; Letícia Chaves de Souza; Gláucia Schuindt Teixeira; Renato M Silva; Robert E W Hancock; Octavio Luiz Franco; Taia Maria Berto Rezende Journal: Bioact Mater Date: 2022-02-01